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AB134177

Anti-IL-15 antibody [EPR1542Y]

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(3 Publications)

Rabbit Recombinant Monoclonal IL-15 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 3 publications.

View Alternative Names

Interleukin-15, IL-15, IL15

1 Images
Western blot - Anti-IL-15 antibody [EPR1542Y] (AB134177)
  • WB

Unknown

Western blot - Anti-IL-15 antibody [EPR1542Y] (AB134177)

All lanes:

Western blot - Anti-IL-15 antibody [EPR1542Y] (ab134177) at 1/1000 dilution

Lane 1:

Recombinant human IL-15 protein at 0.01 µg

Lane 2:

Recombinant human IL-15 protein at 0.005 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 13 kDa

false

  • Carrier free

    Anti-IL-15 antibody [EPR1542Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1542Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8178155, PubMed : 9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed : 9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed : 10233906, PubMed : 23104097, PubMed : 8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed : 7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
See full target information IL15
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40404207

2025

Engineered expressing anti-PD-L1/IL-15 immunocytokine induces and activates specific antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Mei,Junmeng Zhu,Jie Shao,Lin Li,Fangcen Liu,Xiaoxuan Sha,Yang Yang,Jie Shen,Rutian Li,Baorui Liu

Nature communications 13:7658 PubMed36496445

2022

Sirt6 attenuates chondrocyte senescence and osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Liang Ji,Hua Jiang,Zhuang Li,Rui Geng,Jun Zheng Hu,Yu Cheng Lin,Jun Lu

Cellular & molecular immunology 14:293-307 PubMed26364916

2015

Human fused NKG2D-IL-15 protein controls xenografted human gastric cancer through the recruitment and activation of NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Bei Chen,Ti Yang,Weiming Xiao,Li Qian,Yanbing Ding,Mingchun Ji,Xiaoqun Ge,Weijuan Gong
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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