Rabbit Recombinant Monoclonal IL-15 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--western-blot-img8665.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625)")
![Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--western-blot-img12354.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625)")
![Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--immunoprecipitation-img4090.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)")
![Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (AB273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--flow-cytometry-intracellular-img37833.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (AB273625)")
![Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--immunoprecipitation-img3328.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)")
Publications
Filter by
- Nature communications 14:67482023Biomimetic nanovaccine-mediated multivalent IL-15 self-transpresentation (MIST) for potent and safe cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaiyuan Wang,Xuanbo Zhang,Hao Ye,Xia Wang,Zhijin Fan,Qi Lu,Songhao Li,Jian Zhao,Shunzhe Zheng,Zhonggui He,Qianqian Ni,Xiaoyuan Chen,Jin SunPubMed 37875481
- Experimental physiology 107:222-2322022Interleukin-15 receptor subunit alpha regulates interleukin-15 localization and protein expression in skeletal muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShino Yoshida,Taku Fujimoto,Toshimasa Takahashi,Ken Sugimoto,Hiroshi Akasaka,Minoru Tanaka,Yibin Huang,Yukiko Yasunobe,Keyu Xie,Yuri Ohnishi,Tomohiro Minami,Yoichi Takami,Koichi Yamamoto,Hiromi RakugiPubMed 35100657
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Cytokine that stimulates the proliferation of T-lymphocytes (PubMed:8178155). Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA (PubMed:8178155). In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation (PubMed:15123770).
Alternative names
Interleukin-15, IL-15, IL15
Recommended products
Rabbit Recombinant Monoclonal IL-15 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 2 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR22635-214
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--western-blot-img8665.jpg "Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)")
Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 37 seconds.
All lanes: Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625) at 1/1000 dilution
Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 3: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
![Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--western-blot-img12354.jpg "Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)")
Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: Heart (Database: HPA).
Exposure time: 26 seconds.
All lanes: Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625) at 1/1000 dilution
Lane 1: Mouse lymph node tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa
![Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--immunoprecipitation-img4090.jpg "Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)")
Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)
IL-15 was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10 ug
Lane 2: ab273625 IP in Mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab273625 in Mouse spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)
Predicted band size: 18 kDa
Observed band size: 20 kDa
![Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (ab273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--flow-cytometry-intracellular-img37833.jpg "Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (ab273625)")
Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (ab273625)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Mouse PBMC cells labelling IL-15 with ab273625 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625), expandable thumbnail](https://content.abcam.com/products/images/il-15-antibody-epr22635-214-ab273625--immunoprecipitation-img3328.jpg "Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)")
Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)
IL-15 was immunoprecipitated from 0.35 mg Human spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human spleen tissue lysate 10 ug
Lane 2: ab273625 IP in Human spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab273625 in Human spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)
Predicted band size: 18 kDa
Observed band size: 20 kDa
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