AB273625

Anti-IL-15 antibody [EPR22635-214]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Recombinant Monoclonal IL-15 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

Interleukin-15, IL-15, IL15

5 Images

Unknown

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)

IL-15 was immunoprecipitated from 0.35 mg Human spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human spleen tissue lysate 10 ug

Lane 2 : ab273625 IP in Human spleen tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273625 in Human spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Mouse PBMC cells labelling IL-15 with ab273625 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Unknown

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)

IL-15 was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse spleen tissue lysate 10 ug

Lane 2 : ab273625 IP in Mouse spleen tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273625 in Mouse spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Lab

Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 37s

Lab

Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissue : Heart (Database : HPA).

All lanes:

Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 26s

702 Alexa Fluor® 680

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680)
805 Alexa Fluor® 790

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed
HRP

Goat Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22635-214

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8178155, PubMed : 9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed : 9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed : 10233906, PubMed : 23104097, PubMed : 8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed : 7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).

See full target information IL15

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:378 PubMed41044791

2025

Electroacupuncture ameliorates cognitive dysfunction in T2DM rats by modulating astrocytic polarization and aberrant energy metabolism in the hippocampus via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Li,Rui Li,Jiayi Lin,Xiaoli Wu

Biochemistry and biophysics reports 41:101925 PubMed40134939

2025

Vitamin D stimulates Il-15 synthesis in rodent muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Franz Ewendt,Fabienne Drewitz,Michael Althammer,Cosima Eichler,Corinna Brandsch,Stefanie Brey,Thomas H Winkler,Mirja R Wilkens,René St-Arnaud,Marina Kreutz,Gabriele I Stangl

The Journal of experimental medicine 221: PubMed39167075

2024

Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Naijun Miao,Dongqing Cao,Jingsi Jin,Guizhi Ma,Haihui Yu,Junwen Qu,Guiping Li,Caixia Gao,Dong Dong,Fan Xia,Wenwen Li

Science advances 10:eadn5993 PubMed39141732

2024

Remodeling p38 signaling in muscle controls locomotor activity via IL-15.

Applications

Unspecified application

Species

Unspecified reactive species

Cintia Folgueira,Leticia Herrera-Melle,Juan Antonio López,Victor Galvan-Alvarez,Marcos Martin-Rincon,María Isabel Cuartero,Alicia García-Culebras,Phillip A Dumesic,Elena Rodríguez,Luis Leiva-Vega,Marta León,Begoña Porteiro,Cristina Iglesias,Jorge L Torres,Lourdes Hernández-Cosido,Clara Bonacasa,Miguel Marcos,María Ángeles Moro,Jesús Vázquez,Jose A L Calbet,Bruce M Spiegelman,Alfonso Mora,Guadalupe Sabio

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

European journal of histochemistry : EJH 68: PubMed38686889

2024

Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chaojie He,Yi Yu,Feifan Wang,Wudi Li,Hui Ni,Meixiang Xiang

Nature communications 14:6748 PubMed37875481

2023

Biomimetic nanovaccine-mediated multivalent IL-15 self-transpresentation (MIST) for potent and safe cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyuan Wang,Xuanbo Zhang,Hao Ye,Xia Wang,Zhijin Fan,Qi Lu,Songhao Li,Jian Zhao,Shunzhe Zheng,Zhonggui He,Qianqian Ni,Xiaoyuan Chen,Jin Sun

Experimental physiology 107:222-232 PubMed35100657

2022

Interleukin-15 receptor subunit alpha regulates interleukin-15 localization and protein expression in skeletal muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shino Yoshida,Taku Fujimoto,Toshimasa Takahashi,Ken Sugimoto,Hiroshi Akasaka,Minoru Tanaka,Yibin Huang,Yukiko Yasunobe,Keyu Xie,Yuri Ohnishi,Tomohiro Minami,Yoichi Takami,Koichi Yamamoto,Hiromi Rakugi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-15 antibody [EPR22635-214]

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Western blot - Anti-IL-15 antibody [EPR22635-214] (AB273625)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Multiplex Kits

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Product promise