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AB273631

Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal IL-15 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Interleukin-15, IL-15, IL15

5 Images
Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)
  • IP

Unknown

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)

IL-15 was immunoprecipitated from 0.35 mg Human spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Human spleen tissue lysate 10 ug

Lane 2 : ab273625 IP in Human spleen tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273625 in Human spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273625).

All lanes:

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (<a href='/en-us/products/primary-antibodies/il-15-antibody-epr22635-214-ab273625'>ab273625</a>)

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Mouse PBMC cells labelling IL-15 with ab273625 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273625).

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)
  • IP

Unknown

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)

IL-15 was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse spleen tissue lysate 10 ug

Lane 2 : ab273625 IP in Mouse spleen tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273625 in Mouse spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273625).

All lanes:

Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (<a href='/en-us/products/primary-antibodies/il-15-antibody-epr22635-214-ab273625'>ab273625</a>)

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Western blot - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)
  • WB

Lab

Western blot - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)

This data was developed using ab273625, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-15 antibody [EPR22635-214] (<a href='/en-us/products/primary-antibodies/il-15-antibody-epr22635-214-ab273625'>ab273625</a>) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 37s

Western blot - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)
  • WB

Lab

Western blot - Anti-IL-15 antibody [EPR22635-214] - BSA and Azide free (AB273631)

This data was developed using ab273625, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissue : Heart (Database : HPA).

All lanes:

Western blot - Anti-IL-15 antibody [EPR22635-214] (<a href='/en-us/products/primary-antibodies/il-15-antibody-epr22635-214-ab273625'>ab273625</a>) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22635-214

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab273631 is the carrier-free version of ab273625.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 15123770). Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8178155, PubMed : 9326248). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (PubMed : 9326248). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits (PubMed : 10233906, PubMed : 23104097, PubMed : 8026467). Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5 (PubMed : 7568001). In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (By similarity).
See full target information IL15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 40:720-737.e5 PubMed35660135

2022

Exercise-induced engagement of the IL-15/IL-15Rα axis promotes anti-tumor immunity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Kurz,Carolina Alcantara Hirsch,Tanner Dalton,Sorin Alberto Shadaloey,Alireza Khodadadi-Jamayran,George Miller,Sumedha Pareek,Hajar Rajaei,Chirayu Mohindroo,Seyda Baydogan,An Ngo-Huang,Nathan Parker,Matthew H G Katz,Maria Petzel,Emily Vucic,Florencia McAllister,Keri Schadler,Rafael Winograd,Dafna Bar-Sagi
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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