Rabbit Recombinant Monoclonal IL-15RA antibody. Suitable for Flow Cyt, Dot, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (AB273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172946.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (AB273022)")
![Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (AB273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--flow-cytometry-img170163.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (AB273022)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (AB273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166474.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (AB273022)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (AB273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171831.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (AB273022)")
![Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (AB273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--dot-blot-img180118.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (AB273022)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | Dot | WB | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
High-affinity receptor for interleukin-15 (PubMed:8530383). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed:15123770). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed:15123770). Expression of different isoforms may alter or interfere with signal transduction (PubMed:10480910). Isoform 5. Does not bind IL15. Isoform 6. Does not bind IL15. Isoform 7. Does not bind IL15. Isoform 8. Does not bind IL15.
Alternative names
CD215, Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA
Recommended products
Rabbit Recombinant Monoclonal IL-15RA antibody. Suitable for Flow Cyt, Dot, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24784-142
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172946.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes in human colon (PMID: 23039249).The section was incubated with ab273022 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (ab273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--flow-cytometry-img170163.jpg "Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (ab273022)")
Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast, Left)/ A-172(Human brain glioblastoma, Right) cells labelling IL-15RA with ab273022 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control: Daudi (PMID: 8530383). Gated on viable cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166474.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen.The section was incubated with ab273022 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171831.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human skin (PMID: 30021889). The section was incubated with ab273022 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
![Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (ab273022), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-epr24784-142-ab273022--dot-blot-img180118.jpg "Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (ab273022)")
Dot Blot - Anti-IL-15RA antibody [EPR24784-142] (ab273022)
Dot blot analysis of IL-15RA using ab273022 at 1/1000 dilution (0.465 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution.
Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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