Anti-IL-15RA antibody [EPR24784-142] - BSA and Azide free

• Flow Cyt

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Flow Cytometry - Anti-IL-15RA antibody [EPR24784-142] - BSA and Azide free (AB282288)

This data was developed using ab273022, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast, Left)/ A-172 (Human brain glioblastoma, Right) cells labelling IL-15RA with ab273022 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control : Daudi (PMID : 8530383). Gated on viable cells.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] - BSA and Azide free (AB282288)

This data was developed using ab273022, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes in human colon (PMID : 23039249).The section was incubated with ab273022 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] - BSA and Azide free (AB282288)

This data was developed using ab273022, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human skin (PMID : 30021889).The section was incubated with ab273022 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-15RA antibody [EPR24784-142] - BSA and Azide free (AB282288)

This data was developed using ab273022, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling IL-15RA with ab273022 at 1/100 (4.65 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human spleen.The section was incubated with ab273022 for 30 minutes at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

• Dot

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Dot Blot - Anti-IL-15RA antibody [EPR24784-142] - BSA and Azide free (AB282288)

This data was developed using ab273022, the same antibody clone in a different buffer formulation.

Dot blot analysis of IL-15RA using ab273022 at 1/1000 (0.465 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST