Mouse Monoclonal IL-15RA antibody. Suitable for IP, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL15RA.
Images
![Flow Cytometry - Anti-IL-15RA antibody [JM7A4] (AB91270), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-jm7a4-ab91270--flow-cytometry-img125029.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-15RA antibody [JM7A4] (AB91270)")
Publications
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- Journal of immunology (Baltimore, Md. : 1950) 203:676-6852019CD137 (4-1BB) Engagement Fine-Tunes Synergistic IL-15- and IL-21-Driven NK Cell Proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurent Vidard et. al.PubMed 31201235
- Proceedings of the National Academy of Sciences of 101:11082-72004IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyörgy Vámosi et. al.PubMed 15263076
- Immunity 17:537-472002IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSigrid Dubois,Jennifer Mariner,Thomas A Waldmann,Yutaka TagayaPubMed 12433361
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IL15RA. Database link Q13261
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5 µg for 106 Cells | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
High-affinity receptor for interleukin-15 (PubMed:8530383). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed:15123770). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed:15123770). Expression of different isoforms may alter or interfere with signal transduction (PubMed:10480910). Isoform 5. Does not bind IL15. Isoform 6. Does not bind IL15. Isoform 7. Does not bind IL15. Isoform 8. Does not bind IL15.
Alternative names
CD215, Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA
Recommended products
Mouse Monoclonal IL-15RA antibody. Suitable for IP, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL15RA.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IL15RA. Database link Q13261
- Clone number
- JM7A4
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
ab91270 is affinity purified
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Flow Cytometry - Anti-IL-15RA antibody [JM7A4] (ab91270), expandable thumbnail](https://content.abcam.com/products/images/il-15ra-antibody-jm7a4-ab91270--flow-cytometry-img125029.jpg "Flow Cytometry - Anti-IL-15RA antibody [JM7A4] (ab91270)")
Flow Cytometry - Anti-IL-15RA antibody [JM7A4] (ab91270)
ab91270 staining IL-15RA in normal Human PBMCs by Flow cytometry.
Normal Human PBMCs were isolated, and cultured in standard conditions for 3 hours. Non adherent cells were removed from culture, and remaining cells were stimulated with LPS and IFN-? for 24 hours.
Open/green histogram; stained with 0.25 μg of Purified Mouse IgG2b Isotype Control
Closed/blue histogram; stained with 0.25 μg of ab91270.
Detection utilised Biotin Anti-Mouse IgG and SAv-PE.
Cells in the large scatter population were used for analysis.
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