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AB189377

Anti-IL-17A antibody [4K5F6]

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(16 Publications)

Mouse Monoclonal IL-17A antibody. Suitable for Flow Cyt (Intra), IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human IL17A aa 1-100.

View Alternative Names

CTLA8, IL17, IL17A, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

3 Images
Flow Cytometry (Intracellular) - Anti-IL-17A antibody [4K5F6] (AB189377)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-17A antibody [4K5F6] (AB189377)

Intracellular flow cytometric analysis of IL-17A in Human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, labeling IL-17A with ab189377 at 0.1 ug/106 cells (red) Green represents isotype control antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [4K5F6] (AB189377)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody [4K5F6] (AB189377)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labelling IL-17A with ab189377 at 10 μg/ml.

Western blot - Anti-IL-17A antibody [4K5F6] (AB189377)
  • WB

Supplier Data

Western blot - Anti-IL-17A antibody [4K5F6] (AB189377)

All lanes:

Western blot - Anti-IL-17A antibody [4K5F6] (ab189377) at 0.2 µg/mL

Lane 1:

Human full-length recombinant IL-17A protein at 0.1 µg/mL

Lane 2:

Mouse full-length recombinant IL-17A protein at 0.1 µg/mL

Lane 3:

Rat full-length recombinant IL-17A protein at 0.1 µg/mL

Predicted band size: 18 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4K5F6

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human IL17A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q16552

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed : 24120361). Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed : 17911633, PubMed : 18684971, PubMed : 19825828, PubMed : 21350122, PubMed : 24120361, PubMed : 8676080). Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (By similarity). In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines (By similarity). In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells (By similarity). Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance (By similarity). Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation (By similarity). Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection (PubMed : 21350122). Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis (By similarity). As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release (By similarity). In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (By similarity). Involved in antiviral host defense through various mechanisms (By similarity). Enhances immunity against West Nile virus by promoting T cell cytotoxicity (By similarity). May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung (By similarity). Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense (By similarity).
See full target information IL17A

Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:1298 PubMed39809837

2025

Targeted dual biologic therapy for erythroderma of unknown etiology guided by high-parameter peripheral blood immunophenotyping.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah L Cornman,Martin P Alphonse,Arbor Dykema,Alexander L Kollhoff,Kevin K Lee,Jaya Manjunath,Emily Z Ma,Varsha Parthasarathy,Junwen Deng,Thomas Pritchard,Anusha Kambala,Melika Marani,Kayla A Parr,Javid P Mohammed,Madan M Kwatra,Jay H Bream,Won Jin Ho,Shawn G Kwatra

International journal of molecular sciences 25: PubMed39456936

2024

Eutopic and Ectopic Endometrial Interleukin-17 and Interleukin-17 Receptor Expression at the Endometrial-Myometrial Interface in Women with Adenomyosis: Possible Pathophysiology Implications.

Applications

Unspecified application

Species

Unspecified reactive species

Le-Tien Hsu,Pei-Chen Lu,Yi-Wen Wang,Hsien-Ming Wu,I-Ju Chen,Hong-Yuan Huang

Nature communications 15:203 PubMed38172124

2024

Targeting IL-17A enhances imatinib efficacy in Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Yunxuan Li,Zhaona Yang,Wenbin Cao,Ying Liu,Luyao Zhao,Tingting Zhang,Chenxi Zhao,Jinmei Yu,Jiaojiao Yu,Jichao Zhou,Xiaowei Zhang,Ping-Ping Li,Mingzhe Han,Sizhou Feng,Billy Wai-Lung Ng,Zhuo-Wei Hu,Erlie Jiang,Ke Li,Bing Cui

International journal of nanomedicine 18:7267-7285 PubMed38090362

2023

Drug Repositioning of Metformin Encapsulated in PLGA Combined with Photothermal Therapy Ameliorates Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Dae Kyu Kim,Jun Young Park,Youn Joo Kang,Dongwoo Khang

Advanced biology 7:e2300220 PubMed37607110

2023

IL-17A/p38 Signaling Pathway Induces Alveolar Epithelial Cell Pyroptosis and Hyperpermeability in Sepsis-Induced Acute Lung Injury by Activating NLRP3 Inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Lin Zhou,Yi Ran He,Yu Jing Liu,Yi Mei Liu,Li Zhen Xuan,Zhun Yong Gu,Hong Yu He,Min Jie Ju

Science advances 9:eabq0712 PubMed37285421

2023

CD4 T cells aggravate hemorrhagic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel X Shi,Yuwen Xiu,Yan Li,Meng Yuan,Kaibin Shi,Qiang Liu,Xiaoying Wang,Wei-Na Jin

Experimental and therapeutic medicine 25:271 PubMed37206552

2023

Changes in the balance of Th17/Treg cells and oxidative stress markers in patients with HIV‑associated pulmonary tuberculosis who develop IRIS.

Applications

Unspecified application

Species

Unspecified reactive species

Long Wen,Lei Shi,Shan-Shan Wan,Tao Xu,Lei Zhang,Zhi-Guo Zhou

Frontiers in bioengineering and biotechnology 11:1070117 PubMed36815882

2023

Eldecalcitol effectively prevents alveolar bone loss by partially improving Th17/Treg cell balance in diabetes-associated periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruihan Gao,Weidong Zhang,Yujun Jiang,Junzhe Zhai,Jian Yu,Hongrui Liu,Minqi Li

Cancer immunology, immunotherapy : CII 72:1199-1208 PubMed36369379

2022

Somatic ARID1A mutation stratifies patients with gastric cancer to PD-1 blockade and adjuvant chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Gu,Puran Zhang,Jieti Wang,Chao Lin,Hao Liu,He Li,Hongyong He,Ruochen Li,Heng Zhang,Weijuan Zhang

International journal of molecular sciences 23: PubMed35457188

2022

Regulation of Th17/Treg Balance by 27-Hydroxycholesterol and 24S-Hydroxycholesterol Correlates with Learning and Memory Ability in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Shanshan Cui,Ling Hao,Wen Liu,Lijing Wang,Mengwei Ju,Wenjing Feng,Rong Xiao
View all publications

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