Rabbit Recombinant Multiclonal IL-17A antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF, IP, IHC-P and reacts with Recombinant full length protein - Mouse, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
I-ELISA | WB | ICC/IF | IP | IHC-P | Flow Cyt (Intra) | |
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Mouse | Expected | Tested | Tested | Tested | Tested | Not recommended |
Rat | Expected | Tested | Expected | Expected | Not recommended | Not recommended |
Recombinant full length protein - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Rat, Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Recombinant full length protein - Mouse | Dilution info - | Notes - |
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed:18025225, PubMed:19144317, PubMed:26431948). Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed:16200068, PubMed:17911633, PubMed:19144317, PubMed:26431948). Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (PubMed:18025225). In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines (PubMed:18025225, PubMed:27923703). In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells (PubMed:18157131). Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance (PubMed:17372004, PubMed:20364087, PubMed:28709803). Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation (PubMed:17470641, PubMed:23255360). Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis (PubMed:26431948). As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release (PubMed:28709803). In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (PubMed:19144317). Involved in antiviral host defense through various mechanisms (PubMed:21946434, PubMed:26735852, PubMed:27795421). Enhances immunity against West Nile virus by promoting T cell cytotoxicity (PubMed:27795421). May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung (PubMed:21946434). Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense (PubMed:26735852).
Ctla8, Il17, Il17a, Interleukin-17A, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
Rabbit Recombinant Multiclonal IL-17A antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF, IP, IHC-P and reacts with Recombinant full length protein - Mouse, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
This antibody does not cross-react with mouse IL-17F.
Unsuitable for rat IHC-P.
ab318151 is the carrier-free version of Anti-IL-17A antibody [RM1202] ab318150.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-IL-17A antibody [RM1202] ab318150, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-IL-17A antibody [RM1202] ab318150 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Mouse IL-17A,Mouse IL-17F.
Antigen concentration: 1000 ng/ml
This antibody does not cross-react with mouse IL-17F.
This data was developed using Anti-IL-17A antibody [RM1202] ab318150, the same antibody clone in a different buffer formulation.
Expression of IL-17A can be induced by PMA and Ionomycin treatment (PMID 28382171).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IL-17A antibody [RM1202] (Anti-IL-17A antibody [RM1202] ab318150) at 1/1000 dilution
Lane 1: Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 40 µg with NFDM/TBST
Lane 2: EL4 treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours, whole cell lysate at 40 µg with NFDM/TBST
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 14-25 kDa, 36 kDa
Exposure time: 81s
This data was developed using Anti-IL-17A antibody [RM1202] ab318150, the same antibody clone in a different buffer formulation.
The expression profile is consistent with the literature (PMID: 17355969).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IL-17A antibody [RM1202] (Anti-IL-17A antibody [RM1202] ab318150) at 1/1000 dilution
Lane 1: Untreated NR8383 (rat alveolar macrophage) whole cell lysate at 40 µg with NFDM/TBST
Lane 2: NR8383 treated with 50ng/ml PMA, 500ng/ml lonomycin Ca2+Salt and 500ng/ml BFA overnight, whole cell lysate at 40 µg with NFDM/TBST
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25 kDa, 36 kDa
Exposure time: 180s
This data was developed using Anti-IL-17A antibody [RM1202] ab318150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) EL4 (mouse lymphoma T lymphocyte) treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours cell pellet. (B) Untreated EL4 cell pellet. tissue labeling IL-17A with Anti-IL-17A antibody [RM1202] ab318150 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) EL4 treated with 10 nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24 hours cell pellet, weak staining on (B) untreated EL4 cell pellet. The section was incubated with Anti-IL-17A antibody [RM1202] ab318150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-IL-17A antibody [RM1202] ab318150, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling IL-17A with Anti-IL-17A antibody [RM1202] ab318150 at 1/50 (9.96 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing weak staining in EL4 cell line (shown in green). The expression increased after treatment with PMA (50 ng/ml), lonomycin Ca2 + Salt (500 ng/ml) and Brefeldin A (500 ng/ml) overnight (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
This data was developed using Anti-IL-17A antibody [RM1202] ab318150, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling IL-17A with Anti-IL-17A antibody [RM1202] ab318150 at 1/1000 (0.498 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse skin. The section was incubated with Anti-IL-17A antibody [RM1202] ab318150 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
IL-17A was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate with Anti-IL-17A antibody [RM1202] ab318150 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IL-17A antibody [RM1202] ab318150 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate
Lane 2: Anti-IL-17A antibody [RM1202] ab318150 IP in EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL-17A antibody [RM1202] ab318150 in treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate
All lanes: Immunoprecipitation - Anti-IL-17A antibody [RM1202] (Anti-IL-17A antibody [RM1202] ab318150) at 1/30 dilution
All lanes: EL4 (mouse lymphoma T lymphocyte) treated with 10nM PMA, 1uM Ionomycin and 300ng/ml BFA for 24h, whole cell lysate with NFDM/TBST
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 145s
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