Anti-IL-17F antibody [EPR17830-169] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-17F antibody [EPR17830-169] - BSA and Azide free (AB234532)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-fixed EL4 (mouse lymphoma T lymphocyte) treated with 50ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500ng/ml Ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours (Right) / Untreated control (Left) labeling IL17F with ab187059 at 1/60 dilution.

Secondary antibody used Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at a 1/2000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187059).

• IP

Supplier Data

Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] - BSA and Azide free (AB234532)

IL17F was immunoprecipitated from 0.35 mg of EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate with ab187059 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187059 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5,000 dilution.

Lane 1 : EL4 (mouse lymphoma T lymphocyte) treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate 10 μg (Input).
Lane 2 : EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab187059 in EL4 treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin calcium salt for 24 hours, and 500 ng/ml Brefeldin A (BFA) was added for the last 20 hours whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187059).

All lanes:

Immunoprecipitation - Anti-IL-17F antibody [EPR17830-169] (<a href='/en-us/products/primary-antibodies/il-17f-antibody-epr17830-169-ab187059'>ab187059</a>)

Predicted band size: 18 kDa

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