AB245697

Anti-IL-18 antibody [125-2H]

Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal IL-18 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Protein within Human IL18.

View Alternative Names

IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

1 Images

Supplier Data

Western blot - Anti-IL-18 antibody [125-2H] (AB245697)

15% SDS PAGE gel.

Detected by chemiluminescence.

All lanes:

Western blot - Anti-IL-18 antibody [125-2H] (ab245697) at 2 µg/mL

All lanes:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (RIPA buffer) at 35 µg

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

125-2H

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Protein within Human IL18. The exact immunogen used to generate this antibody is proprietary information.

Q14116

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed : 14528293, PubMed : 25500532, PubMed : 37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed : 10653850). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33883744).

See full target information IL18

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40801617

2025

Structure of the Secretory Compartments in Goblet Cells in the Colon and Small Intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander A Mironov,Irina S Sesorova,Pavel S Vavilov,Roberto Longoni,Paola Briata,Roberto Gherzi,Galina V Beznoussenko

Neural regeneration research 20:491-502 PubMed38819062

2024

Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Feng,Juanji Li,Tingting Liu,Shiqi Huang,Xiangliang Chen,Shen Liu,Junshan Zhou,Hongdong Zhao,Ye Hong

ASN neuro 13:17590914211003247 PubMed33853366

2021

LncRNA TUG1 Demethylated by TET2 Promotes NLRP3 Expression, Contributes to Cerebral Ischemia/Reperfusion Inflammatory Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Min Yin,Wei-Ping Chen,Xiao-Ping Yin,Jiang-Long Tu,Na Hu,Zheng-Yu Li

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB223293

Anti-IL-18 antibody [EPR22249-212]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (AB223293)

Primary Antibodies

AB243091

Anti-IL-18 antibody [EPR19954-188]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] (AB243091)

Primary Antibodies

AB222926

Anti-IL-18 antibody [EPR19954] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] - BSA and Azide free (AB222926)

Primary Antibodies

AB243295

Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (AB243295)

Primary Antibodies

AB242022

Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] - BSA and Azide free (AB242022)

Primary Antibodies

AB207324

Anti-IL-18 antibody [EPR19954]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-IL-18 antibody [EPR19954] (AB207324)

Complementary Products

Assay Kits

AB215539

Human IL-18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-18 ELISA Kit (AB215539)

Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

Primary Antibodies

AB263899

Anti-NLRP3 antibody [EPR23094-1]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-NLRP3 antibody [EPR23094-1] (AB263899)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com