Anti-IL-18 antibody (ab191860) is a rabbit polyclonal antibody that is used to detect IL-18 in Western Blot, Flow Cytometry, IHC-P. Suitable for Mouse, Rat samples.
- Over 50 publications
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- Advances in clinical and experimental medicine : official organ Wroclaw Medical University 33:171-1812024Serabelisib regulates GSDMD-mediated pyroptosis, apoptosis and migration of hepatoma cells via the PI3K/Akt/E-cadherin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Li,Zhao Huang,Yuxiang ZhouPubMed 37486695
- Journal of nanobiotechnology 21:4192023Exosomal miR-146a-5p derived from human umbilical cord mesenchymal stem cells can alleviate antiphospholipid antibody-induced trophoblast injury and placental dysfunction by regulating the TRAF6/NF-κB axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingfeng Lv,Yuan Wang,Wei Tian,Yuqiu Liu,Mengqi Gu,Xiaotong Jiang,Yanjun Cai,Ruiheng Huo,Yuchen Li,Lei Li,Xietong WangPubMed 37957714
- Scientific reports 13:128712023Decreased renal expression of PAQR5 is associated with the absence of a nephroprotective effect of progesterone in a rat UUO model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP A Abramicheva,D S Semenovich,L D Zorova,I B Pevzner,I A Sokolov,V A Popkov,E P Kazakov,D B Zorov,E Y PlotnikovPubMed 37553369
- The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 27:345-3562023Hydrogen sulfide ameliorates abdominal aorta coarctation-induced myocardial fibrosis by inhibiting pyroptosis through regulating eukaryotic translation initiation factor 2α phosphorylation and activating PI3K/AKT1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaling Li,Zhixiong Wu,Jiangping Hu,Gongli Liu,Hongming Hu,Fan Ouyang,Jun YangPubMed 37386832
- PloS one 18:e02869032023The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Ding,Sheng Cui,Song Yu Li,Lin Yan Cui,Qi Yan Nan,Xue Jing Lin,Mei Ying Xuan,Jian Jin,Shang Guo Piao,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Byung Ha Chung,Chul Woo Yang,Jing Hao Cui,Can LiPubMed 37310976
- Journal of psychiatry & neuroscience : JPN 48:E154-E1702023Brief pup separation during lactation confers resilience in behavioural deficits induced by chronic restraint stress in postpartum C57BL/6J dams.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Zhou,Zuotian Wu,Dan Zhao,Gaohua Wang,Ling Xiao,Huiling Wang,Yumeng Xie,Limin SunPubMed 37172964
- Antioxidants (Basel, Switzerland) 12:2023Aqueous Extract Ameliorates Cerebral Ischemia/Reperfusion Injury through Inhibiting ROS/NLRP3-Mediated Pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Wu,Jiajia Wei,Yang Yi,Guotao Shu,Zhixu He,Qihai Gong,Jianmei GaoPubMed 37237865
- Toxins 15:2023A Pore Forming Toxin-like Protein Derived from Chinese Red Belly Toad Triggers the Pyroptosis of Hippomal Neural Cells and Impairs the Cognitive Ability of Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqing Ye,Qiquan Wang,Wenhui Lee,Yang Xiang,Jixue Yuan,Yun Zhang,Xiaolong GuoPubMed 36977082
- The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 27:143-1552023Resveratrol pretreatment alleviates NLRP3 inflammasome-mediated cardiomyocyte pyroptosis by targeting TLR4/MyD88/NF-κB signaling cascade in coronary microembolization-induced myocardial damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang-Jun Luo,Tao Li,Hao-Liang Li,You Zhou,Lang LiPubMed 36815254
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 160:1143632023Human placental extract regulates polarization of macrophages via IRGM/NLRP3 in allergic rhinitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeibei Wo,Chunyang Du,Yan Yang,Huimin Qi,Zihui Liang,Conghui He,Fang Yao,Xiaoming LiPubMed 36746096
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen potassium phosphate- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Rat Il18. Database link P97636
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Mouse | Expected | Tested | Expected |
| Rat | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1.00000-3.00000 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.25000-0.50000 µg/mL | Notes - |
Species Mouse | Dilution info 0.25000-0.50000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 2.00000-5.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed:26638072, PubMed:26638073). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (By similarity). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (By similarity). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:30392956).
Alternative names
Igif, Il18, Interleukin-18, IL-18, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma
Recommended products
Anti-IL-18 antibody (ab191860) is a rabbit polyclonal antibody that is used to detect IL-18 in Western Blot, Flow Cytometry, IHC-P. Suitable for Mouse, Rat samples.
- Over 50 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen potassium phosphate- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Rat Il18. Database link P97636
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- reconstitute with water at 0.2mL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-IL-18 antibody (ab191860) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), in Mouse, Rat samples.
What is the molecular weight of IL-18?
Anti-IL-18 (ab191860) specifically detects a band for IL-18 (UniProt: P97636) at a molecular weight of 22kDa.
Recommended positive controls
WB: Mouse spleen, thymus lung and ANA1 whole cell lysatesFlow Cyt: rat RH35 cellsIHC-P: Rat spleen tissue
Trusted by the scientific community
Anti-IL-18 (ab191860) was first used in a scientific publication in 2014 and has been cited over 50 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Flow Cytometry - Anti-IL-18 antibody (ab191860)
Flow Cytometry analysis of RH35 cells stained with ab191860 in blue with a concentration of 1μg/1x106 cells; an isotype control antibody (rabbit IgG) in green with a concentration of 1μg/1x106 cells, and an unlabelled sample in red. The secondary antibody used was DyLight®488 conjugated goat anti-rabbit IgG in the concentration 5-10μg/ 1x106 cells.
Western blot - Anti-IL-18 antibody (ab191860)
SDS PAGE performed on 5-20% gel for 2-3 hours.
Proteins were transferred to a nitrocellulose membrane at 150mA for 50-90 minutes and blocked with 5% milk/TBS for 1.5 hours at room temperature.
All lanes: Western blot - Anti-IL-18 antibody (ab191860) at 0.5 µg/mL
Lane 1: mouse spleen tissue lysates at 30 µg/mL
Lane 2: mouse thymus tissue lysates at 30 µg/mL
Lane 3: mouse lung tissue lysates at 30 µg/mL
Lane 4: mouse ANA-1 whole cell lysates at 30 µg/mL
SecondaryAll lanes: goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody (ab191860)
Immunohistochemistry analysis of paraffin-embedded section of rat spleen tissue labeling IL-18 antibody with ab191860 at 2μg/mL (incubation: overnight at 4°C). Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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