Anti-IL-18 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody (AB191860)

Immunohistochemistry analysis of paraffin-embedded section of mouse lung tissue labeling IL-18 antibody with ab191860 at 2μg/mL. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody ab191860, overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

• WB

Supplier Data

Western blot - Anti-IL-18 antibody (AB191860)

Blocking and diluting buffer and concentration : 5% non-fat milk/TBS

All lanes:

Western blot - Anti-IL-18 antibody (ab191860) at 0.5 µg/mL

Lane 1:

Mouse recombinant IL18 protein lysates, at 30 µg

Lane 2:

Rat PC-12 whole cell lysates at 30 µg

Lane 3:

Rat C6 whole cell lysates at 30 µg

Lane 4:

Mouse RAW264.7 whole cell lysates at 30 µg

Lane 5:

Mouse ANA-1 whole cell lysates at 30 µg

Lane 6:

Mouse J774.1 whole cell lysates at 30 µg

Lane 7:

Mouse ID8 whole cell lysates at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP secondary at 1/5000 dilution

Observed band size: 22 kDa

false

Exposure time: 2hr