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Anti-IL-18 antibody [EPR19956] (ab207323) is a rabbit monoclonal antibody that is used to detect IL-18 in Western Blot, IP. Suitable for Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications


Images

Western blot - Anti-IL-18 antibody [EPR19956] (AB207323), expandable thumbnail
  • Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (AB207323), expandable thumbnail
  • Western blot - Anti-IL-18 antibody [EPR19956] (AB207323), expandable thumbnail
  • Western blot - Anti-IL-18 antibody [EPR19956] (AB207323), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Mouse
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/40
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed:26638072, PubMed:26638073). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (By similarity). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (By similarity). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:30392956).

Alternative names

Igif, Il18, Interleukin-18, IL-18, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

Recommended products

Anti-IL-18 antibody [EPR19956] (ab207323) is a rabbit monoclonal antibody that is used to detect IL-18 in Western Blot, IP. Suitable for Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19956
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-IL-18 antibody [EPR19956] (ab207323) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), in Mouse samples.

What is the molecular weight of IL-18?


Anti-IL-18 [EPR19956] (ab207323) specifically detects a band for IL-18 (UniProt: P70380) at a molecular weight of 22kDa.

Recommended positive controls


WB: Mouse IL-18 active protein; Mouse spleen lysate.IP: Mouse spleen lysate.

Trusted by the scientific community


Anti-IL-18 [EPR19956] (ab207323) was first used in a scientific publication in 2017 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR19956] also available for your convenience:
ab207323, Carrier free - Anti-IL-18 antibody [EPR19956] - BSA and Azide free ab240376



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-IL-18 antibody [EPR19956] (ab207323), expandable thumbnail

    Western blot - Anti-IL-18 antibody [EPR19956] (ab207323)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-IL-18 antibody [EPR19956] (ab207323) at 1/1000 dilution

    All lanes: Mouse IL-18 active protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa, 63 kDa, 72 kDa

    Observed band size: 19 kDa, 63 kDa, 75 kDa

    Exposure time: 1s

  • Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (ab207323), expandable thumbnail

    Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (ab207323)

    IL-18 was immunoprecipitated from 0.35 mg of mouse spleen lysate with ab207323 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse spleen lysate 10μg (Input).

    Lane 2: ab207323 IP in mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207323 in mouse spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (ab207323)

    Predicted band size: 22 kDa

  • Western blot - Anti-IL-18 antibody [EPR19956] (ab207323), expandable thumbnail

    Western blot - Anti-IL-18 antibody [EPR19956] (ab207323)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The protein expression level can refer to reference PMID 10051628.

    All lanes: Western blot - Anti-IL-18 antibody [EPR19956] (ab207323) at 1/1000 dilution

    All lanes: Mouse spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa

    Observed band size: 22 kDa

    Exposure time: 3min

  • Western blot - Anti-IL-18 antibody [EPR19956] (ab207323), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-IL-18 antibody [EPR19956] (ab207323)

    IL-18 western blot using anti-IL-18 antibody [EPR19956] ab207323. Publication image and figure legend from Yang, M., Fang, J. T., et al., 2021, Biomed Res Int, PubMed 34222479.


    ab207323 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab207323 please see the product overview.

    Western blot showed AC-YVAD-CMK significantly inhibits the expression of Caspas-1, NLRP-1, IL-1β, and IL-18 in the renal tissues after cecal ligation and puncture. The same blot was stripped and reprobed with actin to confirm equal loading. ∗P < 0.05 in comparison with the Control group. #P < 0.05 in comparison with the CLP group. N = 6 for each group. The bar represents mean ± s.e.m.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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