Anti-IL-18 antibody [EPR19956]

• IP

Supplier Data

Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (AB207323)

IL-18 was immunoprecipitated from 0.35 mg of mouse spleen lysate with ab207323 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse spleen lysate 10μg (Input).

Lane 2 : ab207323 IP in mouse spleen lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207323 in mouse spleen lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-IL-18 antibody [EPR19956] (ab207323)

Predicted band size: 22 kDa

false

• WB

Supplier Data

Western blot - Anti-IL-18 antibody [EPR19956] (AB207323)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-18 antibody [EPR19956] (ab207323) at 1/1000 dilution

All lanes:

Mouse IL-18 active protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa,21 kDa

Observed band size: 18 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-IL-18 antibody [EPR19956] (AB207323)

Blocking/Dilution buffer : 5% NFDM/TBST.

The protein expression level can refer to reference PMID 10051628.

All lanes:

Western blot - Anti-IL-18 antibody [EPR19956] (ab207323) at 1/1000 dilution

All lanes:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Exposure time: 3min

• WB

CiteAb

Western blot - Anti-IL-18 antibody [EPR19956] (AB207323)

IL-18 western blot using anti-IL-18 antibody [EPR19956] ab207323. Publication image and figure legend from Yang, M., Fang, J. T., et al., 2021, Biomed Res Int, PubMed 34222479.

ab207323 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab207323 please see the product overview.

Western blot showed AC-YVAD-CMK significantly inhibits the expression of Caspas-1, NLRP-1, IL-1β, and IL-18 in the renal tissues after cecal ligation and puncture. The same blot was stripped and reprobed with actin to confirm equal loading. *p < 0.05 in comparison with the Control group. #p < 0.05 in comparison with the CLP group. N = 6 for each group. The bar represents mean ± s.e.m.

false