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AB308442

Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL-18R1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD218a, IL1RRP, IL18R1, Interleukin-18 receptor 1, IL-18R-1, IL-18R1, CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein, Interleukin-18 receptor alpha, IL-1Rrp, IL1R-rp, IL-18R-alpha, IL-18Ralpha

4 Images
Flow Cytometry - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)

This data was developed using ab308441, the same antibody clone in a different buffer formulation. Flow cytometric analysis of THP-1 (human monocytic leukemia monocyte, Left) / HDLM-2 (human Hodgkin lymphoma cell, Right) cells labelling IL-18R1 with ab308441 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : THP-1 (PMID : 10865972). Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)

This data was developed using ab308441, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 80% Methanol-fixed, 0.1% Tween-20 permeabilized HDLM-2(human Hodgkin lymphoma cell) cells labeling IL-18R1 with ab308441 at 1/500 (1.008 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in HDLM-2 cell line.Negative control : THP-1 (PMID : 10865972). The image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)

This data was developed using ab308441, the same antibody clone in a different buffer formulation. Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) treated with 200 ng/ml IL-12 for 6 days cells labeling IL-18R1 with ab308441 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells co-stained with CD3 conjugated to 647. Gated on viable cells.

Flow Cytometry - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-18R1 antibody [EPR26127-35] - BSA and Azide free (AB308442)

This data was developed using ab308441, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte, Left) / U-937 (human histiocytic lymphoma monocyte, Right) cells labelling IL-18R1 with ab308441 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : Ramos (PMID : 10865972). Gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26127-35

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Within the IL18 receptor complex, responsible for the binding of the pro-inflammatory cytokine IL18, but not IL1A nor IL1B (PubMed : 14528293, PubMed : 25261253, PubMed : 25500532, PubMed : 37993714, PubMed : 8626725). Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).
See full target information IL18R1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com