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AB212208

Anti-IL-1R-2 antibody

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(4 Publications)

Rabbit Polyclonal IL-1R-2 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD121b, IL1RB, IL1R2, Interleukin-1 receptor type 2, IL-1R-2, IL-1RT-2, IL-1RT2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, Interleukin-1 receptor type II, IL-1R-beta

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (AB212208)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (AB212208)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney labeling IL-1R-2 with ab212208 at 1/200 dilution overnight at 4°C followed by Goat anti-rabbit IgG (H+L), FITC conjugated at 1/500 dilution for 90 minutes. Nuclei are stained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (AB212208)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (AB212208)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat colon tissue labeling IL-1R-2 with ab212208 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-IL-1R-2 antibody (AB212208)
  • WB

Supplier Data

Western blot - Anti-IL-1R-2 antibody (AB212208)

All lanes:

Western blot - Anti-IL-1R-2 antibody (ab212208) at 1/300 dilution

All lanes:

Mouse lung lysates

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP at 1/5000 dilution

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P27930

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
See full target information IL1R2

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:101228 PubMed37852176

2023

Replenishing decoy extracellular vesicles inhibits phenotype remodeling of tissue-resident cells in inflammation-driven arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Liang,Ke Wang,Xiaoyu Wei,Xiaoshan Gong,Hao Tang,Hao Xue,Jing Wang,Pengbin Yin,Licheng Zhang,Zaisong Ma,Ce Dou,Shiwu Dong,Jianzhong Xu,Fei Luo,Qinyu Ma

Endocrinology 164: PubMed36524678

2022

Decreased Expression of EZH2 in Granulosa Cells Contributes to Endometriosis-Associated Infertility by Targeting IL-1R2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Lin,Xiaomei Tong,Yinli Zhang,Weijia Gu,Qianmeng Huang,Yi Zhang,Feng Zhuo,Fanxuan Zhao,Xiaoying Jin,Chao Li,Dong Huang,Songying Zhang,Yongdong Dai

Frontiers in genetics 13:1036233 PubMed36468014

2022

Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Yuqing,Gao Shang,Gao Qing,Wang Jiyang,Li Ruihao,Chen Zuoguan,Diao Yongpeng,Wu Zhiyuan,Li Yongjun

International journal of molecular medicine 41:2213-2224 PubMed29393354

2018

Effects of silencing the DUSP1 gene using lentiviral vector-mediated siRNA on the release of proinflammatory cytokines through regulation of the MAPK signaling pathway in mice with acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Shu-Liang Li,Hua-Lei Xie,Jia-Wei Fan,Chuan-Wei Gu,Chao Kang,Mu-Jian Teng
View all publications

Product promise

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