Rabbit Polyclonal IL-1R-2 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Expected |
Mouse | Expected | Tested |
Rat | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
IL1RB, IL1R2, Interleukin-1 receptor type 2, IL-1R-2, IL-1RT-2, IL-1RT2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, Interleukin-1 receptor type II, IL-1R-beta
Rabbit Polyclonal IL-1R-2 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Liquid
Polyclonal
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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All lanes: Western blot - Anti-IL-1R-2 antibody (ab212208) at 1/300 dilution
All lanes: Mouse lung lysates
All lanes: Goat anti-rabbit IgG (H+L), HRP at 1/5000 dilution
Predicted band size: 45 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat colon tissue labeling IL-1R-2 with ab212208 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney labeling IL-1R-2 with ab212208 at 1/200 dilution overnight at 4°C followed by Goat anti-rabbit IgG (H+L), FITC conjugated at 1/500 dilution for 90 minutes. Nuclei are stained with DAPI.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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