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Rabbit Polyclonal IL-1R-2 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

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Images

Western blot - Anti-IL-1R-2 antibody (AB212208), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (AB212208), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (AB212208), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P27930

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Expected
Mouse
Expected
Tested
Rat
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

1/100.00000 - 1/500.00000

Notes

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Alternative names

Recommended products

Rabbit Polyclonal IL-1R-2 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human IL1R2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P27930
Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-IL-1R-2 antibody (ab212208), expandable thumbnail

    Western blot - Anti-IL-1R-2 antibody (ab212208)

    All lanes: Western blot - Anti-IL-1R-2 antibody (ab212208) at 1/300 dilution

    All lanes: Mouse lung lysates

    Secondary

    All lanes: Goat anti-rabbit IgG (H+L), HRP at 1/5000 dilution

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (ab212208), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (ab212208)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat colon tissue labeling IL-1R-2 with ab212208 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (ab212208), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1R-2 antibody (ab212208)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney labeling IL-1R-2 with ab212208 at 1/200 dilution overnight at 4°C followed by Goat anti-rabbit IgG (H+L), FITC conjugated at 1/500 dilution for 90 minutes. Nuclei are stained with DAPI.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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