Skip to main content

Rabbit Recombinant Monoclonal IL-1R-2 antibody. Suitable for IP, Flow Cyt, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-IL-1R-2 antibody [EPR24695-36] (AB273025), expandable thumbnail
  • Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (AB273025), expandable thumbnail
  • Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (AB273025), expandable thumbnail
  • Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (AB273025), expandable thumbnail
  • Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (AB273025), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/994
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Alternative names

CD121b, IL1RB, IL1R2, Interleukin-1 receptor type 2, IL-1R-2, IL-1RT-2, IL-1RT2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, Interleukin-1 receptor type II, IL-1R-beta

Recommended products

Rabbit Recombinant Monoclonal IL-1R-2 antibody. Suitable for IP, Flow Cyt, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24695-36
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025), expandable thumbnail

    Flow Cytometry - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left)/ HDLM-2(Human Hodgkin lymphoma, Right) cells labelling IL-1R-2 with ab273025 at 1/50 dilution (1ug) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat. Gated on viable cells.

  • Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025), expandable thumbnail

    Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 26406983).

    Negative control: Jurkat and THP-1 (PMID:8702856).

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 92 seconds

    All lanes: Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025) at 1/1000 dilution

    Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate at 20 µg

    Lane 2: HDLM-2 (human hodgkin lymphoma ) whole cell lysate at 20 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 4: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa

    Observed band size: 55-70 kDa

  • Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025), expandable thumbnail

    Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody specifically recognizes IL1R2 recombinant protein, but not IL1R1 recombinant protein.

    Exposure time: 1 second

    All lanes: Western blot - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025) at 1/1000 dilution

    Lane 1: His-tagged human IL1R1 recombinant protein (aa 18-332) at 0.02 µg

    Lane 2: His-tagged human IL1R2 recombinant protein (aa 14-343) at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa

  • Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025), expandable thumbnail

    Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    IL-1R-2 was immunoprecipitated from 0.35 mg SR (human pleural effusion lymphoblast) whole cell lysate 10 ug with ab273025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273025 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SR (human pleural effusion lymphoblast) whole cell lysate 10 ug

    Lane 2: ab273025 IP in SR whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab273025 in SR whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.

    All lanes: Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    Predicted band size: 45 kDa

    Observed band size: 50-70 kDa

  • Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025), expandable thumbnail

    Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    IL-1R-2 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 ug with ab273025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273025 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 ug

    Lane 2: ab273025 IP in HDLM-2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab273025 in HDLM-2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.

    All lanes: Immunoprecipitation - Anti-IL-1R-2 antibody [EPR24695-36] (ab273025)

    Predicted band size: 45 kDa

    Observed band size: 50-70 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com