Anti-IL-1RA antibody [EPR29922-572]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling IL-1RA with ab324456 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse skin.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling IL-1RA with ab324456 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse colon.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded Mouse squamous cell carcinoma tissue labeling IL-1RA with ab324456 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse squamous cell carcinoma.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue labeling IL-1RA with ab324456 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse esophagus.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9597123; PMID : 37563620; PMID : 38356712).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL-1RA antibody [EPR29922-572] (ab324456) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 48 µg

Lane 2:

RAW 264.7 treated with 50 ng/ml TNF alpha for 24 hours whole cell lysate at 48 µg

Lane 3:

Mosue colon tissue lysate at 48 µg

Lane 4:

Mouse spleen tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,20 kDa,37 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : CTLL-2, EL4

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9597123; PMID : 37563620; PMID : 38356712).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL-1RA antibody [EPR29922-572] (ab324456) at 1/1000 dilution

Lane 1:

NMuLi (mouse liver epithelial cell) whole cell lysate at 40 µg

Lane 2:

CTLL-2 (mouse T lymphocyte) whole cell lysate at 40 µg

Lane 3:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,20 kDa,37 kDa

false

Exposure time: 10s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling IL-1RA with ab324456 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse kidney.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse IL-1RA expression vector containing a myc-His-tag, (B) HEK-293T cells transfected with a mouse IL-36A expression vector containing a myc-His-tag, (C) HEK-293T cells transfected with a mouse IL-36B expression vector containing a myc-His-tag, (D) HEK-293T cells transfected with a mouse IL-36RA expression vector containing a myc-His-tag, (E) HEK-293T cells transfected with empty vector containing a myc-His-tag, labeling IL-1RA with ab324456 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse IL-1RA expression vector containing a myc-His-tag. Negative staining on (B) HEK-293T cells transfected with a mouse IL-36A expression vector containing a myc-His-tag, (C) HEK-293T cells transfected with a mouse IL-36B expression vector containing a myc-His-tag, (D) HEK-293T cells transfected with a mouse IL-36RA expression vector containing a myc-His-tag, (E) HEK-293T cells transfected with empty vector containing a myc-His-tag.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1RA antibody [EPR29922-572] (AB324456)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling IL-1RA with ab324456 at 1/1000 (0.503 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen.
The section was incubated with ab324456 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.