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Knockout Tested Rabbit Recombinant Monoclonal IL-1RA antibody. Suitable for WB and reacts with Human samples. Cited in 33 publications.


Images

Western blot - Anti-IL-1RA antibody [EPR6483] (AB124962), expandable thumbnail
  • OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (AB124962), expandable thumbnail
  • Western blot - Anti-IL-1RA antibody [EPR6483] (AB124962), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

Observed band at >20 kDa is likely an unrelated protein.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

Alternative names

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, IL1RN, IL1F3, IL1RA

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal IL-1RA antibody. Suitable for WB and reacts with Human samples. Cited in 33 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR6483

Purification technique

Affinity purification Protein A

Dissociation constant

8.4 x 10-12 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962), expandable thumbnail

    Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)

    False colour image of Western blot: Anti-IL-1RA antibody [EPR6483] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124962 was shown to bind specifically to IL-1RA. A band was observed at 19 kDa in wild-type A431 cell lysates with no signal observed at this size in IL1RN knockout cell line Human IL1RN (IL1 Receptor Antagonist) knockout A-431 cell line ab273379 (knockout cell lysate Human IL1RN (IL1 Receptor Antagonist) knockout A-431 cell lysate ab275530). To generate this image, wild-type and IL1RN knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962) at 1/10000 dilution

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: IL-1RA knockout A431 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 20 kDa

    Observed band size: 19 kDa

  • OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962), expandable thumbnail

    OI-RD Scanning - Anti-IL-1RA antibody [EPR6483] (ab124962)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962), expandable thumbnail

    Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962)

    False colour image of Western blot: Anti-IL-1RA antibody [EPR6483] staining at 1/50000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124962 was shown to bind specifically to IL-1RA. A band was observed at 18 kDa in wild-type A431 cell lysates with no signal observed at this size in IL1RN knockout cell line Human IL1RN (IL1 Receptor Antagonist) knockout A-431 cell line ab273379 (knockout cell lysate Human IL1RN (IL1 Receptor Antagonist) knockout A-431 cell lysate ab275530). To generate this image, wild-type and IL1RN knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-IL-1RA antibody [EPR6483] (ab124962) at 1/50000 dilution

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: IL1RN knockout A431 cell lysate at 20 µg

    Lane 3: MOLT-4 cell lysate at 20 µg

    Lane 4: Human Kidney cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 18 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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