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AB256764

Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal IL-1RAcP antibody. Carrier free. Suitable for IP, Flow Cyt, I-ELISA, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.

View Alternative Names

C3orf13, IL1R3, IL1RAP, Interleukin-1 receptor accessory protein, IL-1 receptor accessory protein, IL-1RAcP, Interleukin-1 receptor 3, IL-1R-3, IL-1R3

4 Images
Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)
  • Flow Cyt

Lab

Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)

This data was developed using the same antibody clone in a different buffer formulation (ab256461)

Flow cytometry overlay histogram showing wild-type HeLa (green line) and IL1RAP knockout HeLa cells (ab273375) stained with ab256461 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab256461) (1x106 in 100μl at 10 μ/ml) for 30 min at 4°C.

The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.

Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line; IL1RAP knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)
  • Flow Cyt

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Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)

Flow cytometric analysis of Raji (Human burkitt's lymphoma b lymphocyte, Left panel) and HepG2 (Human hepatocellular carcinoma epithelial cell, Right panel) cells labeling IL-1RAcP with ab256461 at a 1/60 dilution (1μg, red), compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Negative control : Raji (PMID : 30514753). Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256461).

Indirect ELISA - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)
  • I-ELISA

Lab

Indirect ELISA - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)

This data was developed using ab256461, the same antibody clone in a different buffer formulation.ELISA analysis of Hu IL1RAP recombinant protein at 1000 ng/mL with ab256461. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)
  • IP

Unknown

Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (AB256764)

IL-1RAcP was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg with ab256461 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256461 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg.

Lane 2 : ab256461 IP in KARPAS-299 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256461 in KARPAS-299 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 75 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256461).

All lanes:

Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] (<a href='/en-us/products/primary-antibodies/il-1racp-antibody-epr22678-67-ab256461'>ab256461</a>)

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22678-67

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

I-ELISA, WB, IP, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab256764 is the carrier-free version of ab256461.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable).. Isoform 2. Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (PubMed : 12530978). Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity).. Isoform 4. Unable to mediate canonical IL-1 signaling (PubMed : 19481478). Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons (By similarity).
See full target information IL1RAP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 20:e13454 PubMed34510683

2021

microRNA-425 loss mediates amyloid plaque microenvironment heterogeneity and promotes neurodegenerative pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Bo Hu,Yong-Fang Zhang,Ru-Jing Ren,Eric B Dammer,Xin-Yi Xie,Shi-Wu Chen,Qiang Huang,Wan-Ying Huang,Rui Zhang,Hong-Zhuan Chen,Hao Wang,Gang Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com