Rabbit Recombinant Monoclonal IL-1RAcP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (AB323912), expandable thumbnail](https://content.abcam.com/products/images/il-1racp-antibody-epr26778-33-bsa-and-azide-free-detector-ab323912--sandwich-elisa-img445820.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (AB323912)")
![Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (AB323912), expandable thumbnail](https://content.abcam.com/products/images/il-1racp-antibody-epr26778-33-bsa-and-azide-free-detector-ab323912--sandwich-elisa-img445819.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (AB323912)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes - |
Target data
Function
Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable). Isoform 2. Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (PubMed:12530978). Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity). Isoform 4. Unable to mediate canonical IL-1 signaling (PubMed:19481478). Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons (By similarity).
Alternative names
C3orf13, IL1R3, IL1RAP, Interleukin-1 receptor accessory protein, IL-1 receptor accessory protein, IL-1RAcP, Interleukin-1 receptor 3, IL-1R-3, IL-1R3
Rabbit Recombinant Monoclonal IL-1RAcP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26778-33
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323912 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify IL-1RAcP. The recommended pair for sandwich ELISA is:
Capture: Anti-IL-1RAcP antibody [EPR26778-10] – BSA and Azide free (Capture) ab323911, IL-1RAcP Capture Antibody (unconjugated)
Detector: ab323912, IL-1RAcP Detector Antibody (unconjugated)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (ab323912), expandable thumbnail](https://content.abcam.com/products/images/il-1racp-antibody-epr26778-33-bsa-and-azide-free-detector-ab323912--sandwich-elisa-img445820.jpg "Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (ab323912)")
Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (ab323912)
Interpolated concentration of IL1RAP was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Neat Human Serum and Human Plasma. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). We do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (ab323912), expandable thumbnail](https://content.abcam.com/products/images/il-1racp-antibody-epr26778-33-bsa-and-azide-free-detector-ab323912--sandwich-elisa-img445819.jpg "Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (ab323912)")
Sandwich ELISA - Anti-IL-1RAcP antibody [EPR26778-33] – BSA and Azide free (Detector) (ab323912)
Sandwich ELISA using Human IL1RAP immunogen protein standard curve with the capture antibody Anti-IL-1RAcP antibody [EPR26778-10] – BSA and Azide free (Capture) ab323911 dilution at 2 µg/mL and detector antibody ab323912 dilution at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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