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Rabbit Recombinant Monoclonal IL-2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples.


Images

Western blot - Anti-IL-2 antibody [EPR20055] (AB207325), expandable thumbnail
  • Western blot - Anti-IL-2 antibody [EPR20055] (AB207325), expandable thumbnail
  • Immunoprecipitation - Anti-IL-2 antibody [EPR20055] (AB207325), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR20055] (AB207325), expandable thumbnail
  • Western blot - Anti-IL-2 antibody [EPR20055] (AB207325), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

2 products for Alternative Version

Target data

Function

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:6438535). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed:16293754, PubMed:16477002). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed:7973659). In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:8580378). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed:25142963). Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed:6608729). Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed:6438535). Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.

Alternative names

Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2

Recommended products

Rabbit Recombinant Monoclonal IL-2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20055
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-IL-2 antibody [EPR20055] (ab207325), expandable thumbnail

    Western blot - Anti-IL-2 antibody [EPR20055] (ab207325)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human IL2 active recombinant protein contains aa21-153.

    All lanes: Western blot - Anti-IL-2 antibody [EPR20055] (ab207325) at 1/1000 dilution

    All lanes: Human IL2 active recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa

    Observed band size: 14 kDa

    Exposure time: 1min

  • Western blot - Anti-IL-2 antibody [EPR20055] (ab207325), expandable thumbnail

    Western blot - Anti-IL-2 antibody [EPR20055] (ab207325)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with the literature, and the expression level can be induced in activated Jurkat cell. (PMID:10555752).

    All lanes: Western blot - Anti-IL-2 antibody [EPR20055] (ab207325) at 1/5000 dilution

    Lane 1: Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa

    Observed band size: 14 kDa

    Exposure time: 1min

  • Immunoprecipitation - Anti-IL-2 antibody [EPR20055] (ab207325), expandable thumbnail

    Immunoprecipitation - Anti-IL-2 antibody [EPR20055] (ab207325)

    IL2 was immunoprecipitated from 0.35 mg of Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate with ab207325 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab207325 at 1/200 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Jurkat treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate 10μg (Input).

    Lane 2: ab207325 IP in Jurkat treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207325 in Jurkat treated with 40nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-IL-2 antibody [EPR20055] (ab207325)

    Predicted band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR20055] (ab207325), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR20055] (ab207325)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells, untreated or treated with 40 nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight, labeling IL2 with ab207325 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on Jurkat cells. The signal is induced after treatment with 40 nM Phorbol-12-myristate-13-acetate, 2 μM A23187, and 300 ng/ml Brefeldin A (45 min delay) overnight.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only controls on non treated and treated cells: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Western blot - Anti-IL-2 antibody [EPR20055] (ab207325), expandable thumbnail

    Western blot - Anti-IL-2 antibody [EPR20055] (ab207325)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    ab207325 is recommended to detect endogenous IL-2.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as GAPDH loading control.

    Lanes 1 - 2: Western blot - Anti-IL-2 antibody [EPR2780] (Anti-IL-2 antibody [EPR2780] ab92381) at 1/5000 dilution

    Lanes 1 - 2: Western blot - Anti-IL-2 antibody [EPR20055] (ab207325)

    Lane 1: Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

    Lane 2: Jurkat cells treated with 40nM Phorbol-12-myristate-13-acetate,2 µM A23187 and 300 ng/ml Brefeldin A (45 min delay) overnight whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa

    Observed band size: 14 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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