Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) - BSA and Azide Free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) - BSA and Azide Free (AB306566)

This data was developed using ab306565, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labeling IL-2 with ab306565 at 1/100 dilution, followed by ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing cytoplasmic staining in Jurkat cells treated with TPA (40 nM) and A23187 (2 uM) for 1 h, then together with BFA (300 ng/ml) for another 23 h. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

• WB

Supplier Data

Western blot - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) - BSA and Azide Free (AB306566)

This data was developed using ab306565, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with the literature (PMID : 10555752). Exposure time : 180 seconds.

All lanes:

Western blot - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/il-2-antibody-epr20055-chicken-igy-chimeric-ab306565'>ab306565</a>) at 1/1000 dilution

Lane 1:

Untreated Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

Jurkat treated with 40nM TPA (<a href='/en-us/products/biochemicals/phorbol-12-myristate-13-acetate-pma-pkc-activator-ab120297'>ab120297</a>) and 2 µM A23187 (<a href='/en-us/products/biochemicals/a23187-calcimycin-ca2-ionophore-ab120287'>ab120287</a>) for 24 hours, and /ml Brefeldin A at last 23 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/10000 dilution

Observed band size: 17 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) - BSA and Azide Free (AB306566)

This data was developed using ab306565, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 6 seconds

All lanes:

Western blot - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/il-2-antibody-epr20055-chicken-igy-chimeric-ab306565'>ab306565</a>) at 1/1000 dilution

All lanes:

Human IL-2 recombinant protein fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-ab6877'>ab6877</a>) at 1/10000 dilution

Observed band size: 17 kDa

false

Exposure time: 6s