Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (AB246689)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92381). Immunocytochemistry/ Immunofluorescence analysis of PBMC (Human primary peripheral blood mononuclear cell) cells labeling IL-2 using ab92381. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab92381 at 1 : 50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (shown in red). Secondary antibody only control : PBS instead of the primary antibody.

• WB

Lab

Western blot - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (AB246689)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92381). Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab207325 is recommended to detect endogenous IL-2. ab181602 was used as GAPDH loading control.

Lanes 1 - 2:

Western blot - Anti-IL-2 antibody [EPR2780] (<a href='/en-us/products/primary-antibodies/il-2-antibody-epr2780-ab92381'>ab92381</a>) at 1/5000 dilution

Lanes 1 - 2:

Western blot - Anti-IL-2 antibody [EPR20055] (<a href='/en-us/products/primary-antibodies/il-2-antibody-epr20055-ab207325'>ab207325</a>)

Lane 1:

Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 2:

Jurkat cells treated with 40nM Phorbol-12-myristate-13-acetate,2 µM A23187 and 300 ng/ml Brefeldin A (45 min delay) overnight whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 17 kDa

Observed band size: 14 kDa

false

• WB

Lab

Western blot - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (AB246689)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92381). Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-2 antibody [EPR2780] (<a href='/en-us/products/primary-antibodies/il-2-antibody-epr2780-ab92381'>ab92381</a>) at 1/1000 dilution

All lanes:

Recombinant human IL-2 full length protein, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 15 kDa

false