Rabbit Recombinant Multiclonal IL-2 antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF, IP and reacts with Recombinant full length protein - Human, Recombinant full length protein - Mouse, Human, Mouse samples.
Images
![Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunoprecipitation-img433398.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433357.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433356.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433370.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433371.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | WB | ICC/IF | IP | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Expected | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human, Recombinant full length protein - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Alternative names
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2
Recommended products
Rabbit Recombinant Multiclonal IL-2 antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF, IP and reacts with Recombinant full length protein - Human, Recombinant full length protein - Mouse, Human, Mouse samples.
Alternative names
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
RM1124
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab317332 is the carrier-free version of Anti-IL-2 antibody [RM1124] ab317331.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunoprecipitation-img433398.jpg "Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
IL-2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate with Anti-IL-2 antibody [RM1124] ab317331 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IL-2 antibody [RM1124] ab317331 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 2: Anti-IL-2 antibody [RM1124] ab317331 IP in Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL-2 antibody [RM1124] ab317331 in Jurkat whole cell lysateAll lanes: Immunoprecipitation - Anti-IL-2 antibody [RM1124] (Anti-IL-2 antibody [RM1124] ab317331) at 1/30 dilution
All lanes: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433357.jpg "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
The expression of IL-2 is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).
The identity of the higher MW bands at approximately 50kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IL-2 antibody [RM1124] (Anti-IL-2 antibody [RM1124] ab317331) at 1/1000 dilution
Lane 1: Untreated EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg
Lane 2: EL4.IL-2 cell line treated with Cell Stimation Cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 17 kDa, 36 kDa
Exposure time: 103s
The expression of IL-2 is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).
The identity of the higher MW bands at approximately 50kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433356.jpg "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
The expression of IL-2 is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IL-2 antibody [RM1124] (Anti-IL-2 antibody [RM1124] ab317331) at 1/1000 dilution
Lane 1: Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 2: Jurkat treated with 15nM 24h PMA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 1µM ionomycin (Ionomycin Ca2+ Salt, Ca2+ ionophore ab120116) and 300ng/ml BFA for 24 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 15 kDa, 36 kDa
Exposure time: 180s
The expression of IL-2 is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433370.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling IL-2 with Anti-IL-2 antibody [RM1124] ab317331 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic staining in jurkat cell line (shown in green) treated with 12-O-Tetradecanoylphorbol-13-acetate (15 nM), ionomycin (1 μm) and Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Left Panel is applied with Anti-IL-2 antibody [RM1024] (Anti-IL-2 antibody [RM1124] ab317331) at 1/50 dilution (10 ug/ml); Right panel is applied with Anti-IL-2 antibody (Anti-IL-2 antibody ab9618) at 1/100 dilution (10 ug/ml).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433371.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling IL-2 with Anti-IL-2 antibody [RM1124] ab317331 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic staining in EL4 cells (shown in green) treated with TPA (40 nM), A23187 (2 uM) and BFA (300 ng/ml) for 23 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
![Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--indirect-elisa-img433425.jpg "Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-IL-2 antibody [RM1124] ab317331 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human IL-2,Mouse IL-2.
Antigen concentration: 1000 ng/ml
![Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--indirect-elisa-img433425.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
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