Rabbit Recombinant Multiclonal IL-2 antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF, IP and reacts with Recombinant full length protein - Human, Recombinant full length protein - Mouse, Human, Mouse samples.
Images
![Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunoprecipitation-img433398.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433357.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433356.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433370.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433371.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (AB317332)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | WB | ICC/IF | IP | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Expected | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human, Recombinant full length protein - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:6438535). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed:16293754, PubMed:16477002). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed:7973659). In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:8580378). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed:25142963). Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed:6608729). Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed:6438535). Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
Additional Targets
Il2
Alternative names
Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2
Recommended products
Rabbit Recombinant Multiclonal IL-2 antibody. Carrier free. Suitable for I-ELISA, WB, ICC/IF, IP and reacts with Recombinant full length protein - Human, Recombinant full length protein - Mouse, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1124
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317332 is the carrier-free version of Anti-IL-2 antibody [RM1124] ab317331.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunoprecipitation-img433398.jpg "Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Immunoprecipitation - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
IL-2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate with Anti-IL-2 antibody [RM1124] ab317331 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IL-2 antibody [RM1124] ab317331 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 2: Anti-IL-2 antibody [RM1124] ab317331 IP in Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL-2 antibody [RM1124] ab317331 in Jurkat whole cell lysateAll lanes: Immunoprecipitation - Anti-IL-2 antibody [RM1124] (Anti-IL-2 antibody [RM1124] ab317331) at 1/30 dilution
All lanes: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate with NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433357.jpg "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
The expression of IL-2 is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).
The identity of the higher MW bands at approximately 50kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IL-2 antibody [RM1124] (Anti-IL-2 antibody [RM1124] ab317331) at 1/1000 dilution
Lane 1: Untreated EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: EL4.IL-2 cell line treated with Cell Stimation Cocktail (80nM PMA + 1.34uM Ionomycin + 10.6uM Brefeldin A + 2uM Monensin) for 6 hours whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 17 kDa, 36 kDa
Exposure time: 103s
![Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--western-blot-img433356.jpg "Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Western blot - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
The expression of IL-2 is upregulated in response to PMA/ionomycin treatment (PMID: 23494519).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-IL-2 antibody [RM1124] (Anti-IL-2 antibody [RM1124] ab317331) at 1/1000 dilution
Lane 1: Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: Jurkat treated with 15nM 24h PMA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 1µM ionomycin (Ionomycin Ca2+ Salt, Ca2+ ionophore ab120116) and 300ng/ml BFA for 24 hours whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 15 kDa, 36 kDa
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433370.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling IL-2 with Anti-IL-2 antibody [RM1124] ab317331 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic staining in jurkat cell line (shown in green) treated with 12-O-Tetradecanoylphorbol-13-acetate (15 nM), ionomycin (1 μm) and Brefeldin A (300 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Left Panel is applied with Anti-IL-2 antibody [RM1024] (Anti-IL-2 antibody [RM1124] ab317331) at 1/50 dilution (10 ug/ml); Right panel is applied with Anti-IL-2 antibody (Anti-IL-2 antibody ab9618) at 1/100 dilution (10 ug/ml).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--immunocytochemistry-immunofluorescence-img433371.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling IL-2 with Anti-IL-2 antibody [RM1124] ab317331 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic staining in EL4 cells (shown in green) treated with TPA (40 nM), A23187 (2 uM) and BFA (300 ng/ml) for 23 hr. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
![Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--indirect-elisa-img433425.jpg "Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)
This data was developed using Anti-IL-2 antibody [RM1124] ab317331, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-IL-2 antibody [RM1124] ab317331 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human IL-2,Mouse IL-2.
Antigen concentration: 1000 ng/ml
![Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332), expandable thumbnail](https://content.abcam.com/products/images/il-2-antibody-rm1124-bsa-and-azide-free-ab317332--indirect-elisa-img433425.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-IL-2 antibody [RM1124] - BSA and Azide free (ab317332)")
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