Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for ICC, Flow Cyt, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL2RA aa 1-250.
Images
![Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--immunoprecipitation-img420577.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)")
![Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--immunocytochemistry-img420576.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)")
![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--flow-cytometry-img420575.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)")
![Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--western-blot-img420574.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human IL2RA aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P01589
Reactivity data
Select an application| ICC | Flow Cyt | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Alternative names
CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA
Recommended products
Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for ICC, Flow Cyt, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL2RA aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human IL2RA aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P01589
- Clone number
- BLR157J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--immunoprecipitation-img420577.jpg "Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)")
Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)
Samples: Whole cell lysate (0.5 mg per IP reaction; 5% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J] used for IP at 20 µl/mg lysate. IL-2 Receptor alpha was also immunoprecipitated by another antibody against a different epitope of IL-2 Receptor alpha. For blotting immunoprecipitated IL-2 Receptor alpha, A700-157 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
![Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--immunocytochemistry-img420576.jpg "Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)")
Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)
Detection of human IL-2 Receptor alpha in FFPE MJ cells by immunocytochemistry. Antibody: Rabbit anti-IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J]. Secondary: HRP-conjugated goat antirabbit IgG (A120-501P). Substrate: DAB.
![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--flow-cytometry-img420575.jpg "Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)")
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)
Detection of human IL-2 Receptor alpha (shaded) in MJ cells by flow cytometry.
Antibody: Rabbit anti-IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J] (A700-157CF) or isotype control (unshaded). Secondary: 488-conjugated goat anti-rabbit IgG .![Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-blr157j-bsa-free-ab314083--western-blot-img420574.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)")
Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083)
Samples: Whole cell lysate (25 µg) from MJ, SR, SU-DHL-1, HEK293T, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J] (A700-157CF) used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083) at 1/1000 dilution
Lane 1: MJ Whole cell lysate at 25 µg
Lane 2: SR Whole cell lysate at 25 µg
Lane 3: SU-DHL-1 Whole cell lysate at 25 µg
Lane 4: HEK293T Whole cell lysate at 25 µg
Lane 5: C5/MJ Whole cell lysate at 25 µg
SecondaryAll lanes: HRP-conjugated goat antirabbit IgG
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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