AB314083

Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for ICC, Flow Cyt, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL2RA aa 1-250.

View Alternative Names

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

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Supplier Data

Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Detection of human IL-2 Receptor alpha in FFPE MJ cells by immunocytochemistry. Antibody : Rabbit anti-IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J]. Secondary : HRP-conjugated goat antirabbit IgG (A120-501P). Substrate : DAB.

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Detection of human IL-2 Receptor alpha (shaded) in MJ cells by flow cytometry. Antibody : Rabbit anti-IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J] (A700-157CF) or isotype control (unshaded). Secondary : 488-conjugated goat anti-rabbit IgG .

Supplier Data

Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Samples : Whole cell lysate (0.5 mg per IP reaction; 5% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies : Rabbit anti IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J] used for IP at 20 µl/mg lysate. IL-2 Receptor alpha was also immunoprecipitated by another antibody against a different epitope of IL-2 Receptor alpha. For blotting immunoprecipitated IL-2 Receptor alpha, A700-157 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

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Supplier Data

Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Samples : Whole cell lysate (25 µg) from MJ, SR, SU-DHL-1, HEK293T, and C5/MJ cells prepared using NETN lysis buffer. Antibody : Rabbit anti-IL-2 Receptor alpha recombinant monoclonal antibody [BLR157J] (A700-157CF) used at 1 : 1000. Secondary : HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (ab314083) at 1/1000 dilution

Lane 1:

MJ Whole cell lysate at 25 µg

Lane 2:

SR Whole cell lysate at 25 µg

Lane 3:

SU-DHL-1 Whole cell lysate at 25 µg

Lane 4:

HEK293T Whole cell lysate at 25 µg

Lane 5:

C5/MJ Whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat antirabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR157J

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, IP, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human IL2RA aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P01589

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information IL2RA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free

Immunocytochemistry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Western blot - Anti-IL-2 Receptor alpha antibody [BLR157J] - BSA free (AB314083)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise