AB255732

Anti-IL-2 Receptor alpha antibody [EPR22588-118]

20ul selling size
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55

3 Images

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Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (AB255732)

IL-2 Receptor alpha was immunoprecipitated from 0.35 mg of WEHI-231 (mouse lymphoblast B cell lymphoma cell line) whole cell lysate with ab255732 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab255732 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

Lane 1 : WEHI-231 whole cell lysate 10 μg (Input).
Lane 2 : ab255732 IP in WEHI whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255732 in WEHI whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 15 seconds.

IL-2 Receptor alpha is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID : 17475646).

All lanes:

Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (ab255732)

Predicted band size: 31 kDa

Observed band size: 50 kDa

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Western blot - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (AB255732)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 3 minutes; Lane 2 : 48 seconds.

IL-2 Receptor alpha is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID : 17475646).

All lanes:

Western blot - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (ab255732) at 1/1000 dilution

Lane 1:

EL4 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg

Lane 2:

WEHI-231 (mouse lymphoblast B cell lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

Observed band size: 50 kDa

false

Unknown

Western blot - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (AB255732)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (ab255732) at 1/1000 dilution

All lanes:

LIF- and His-tagged mouse IL-2 Receptor recombinant protein (aa 22-236), 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

false

Exposure time: 3min

805 Alexa Fluor® 790

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed
HRP

Goat Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22588-118

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information Il2ra

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39312740

2024

Egfl6 promotes ovarian cancer progression by enhancing the immunosuppressive functions of tumor-associated myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Hamze Sinno,Joshua A Imperatore,Shoumei Bai,Noémie Gomes-Jourdan,Nyasha Mafarachisi,Claudia Coronnello,Linan Zhang,Eldin Jašarević,Hatice U Osmanbeyoglu,Ronald J Buckanovich,Sandra Cascio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-2 Receptor alpha antibody [EPR22588-118]

Immunoprecipitation - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (AB255732)

Western blot - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (AB255732)

Western blot - Anti-IL-2 Receptor alpha antibody [EPR22588-118] (AB255732)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise