AB227834

Anti-IL-2 Receptor alpha antibody [EPR22588-18]

Name: Anti-IL-2 Receptor alpha antibody [EPR22588-18] (ab227834) | Abcam
Brand: Abcam Limited
SKU: AB227834
Price: 195 USD
Availability: InStock
Rating: 5 (2 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IL-2 Receptor alpha with ab227834 at 1/500 dilution (1.26 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on T cells of rat spleen (PMID : 17056509) is observed. The section was incubated with ab227834 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IL-2 Receptor alpha with ab227834 at 1/500 dilution (1.26 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on T cells of mouse spleen (PMID : 17056509) is observed. The section was incubated with ab227834 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

AbReview79362****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed mouse CT26 tumor taken out of Balb/C mice tissue. Stained with ab227834 at 1/500 dilution. Secondary antibody used was ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detection. Blocking was done with Peroxidase Blocking Solution, BLOXALL for 20 minutes at 20°C. The sample was incubated with the primary antibody and Signalstain ab diluent for 18 hours at 4°C. Antigen retrieval method was heat mediated with Tris-EDTA pH 9.

This image is courtesy of an anonymous Abreview

Carrier free

Anti-IL-2 Receptor alpha antibody [EPR22588-18] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22588-18

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information Il2ra

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 106:e70169 PubMed40944385

2025

Kisspeptin-10 Improves Gestational Diabetes Mellitus Symptoms in Rats by Suppressing Insulin Resistance in Placental Trophoblast Cells by Activating the Cyclic AMP/Protein Kinase A Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Li,Jinhuan Chen,Lin Lu,Bei Gan

Cell reports 43:114819 PubMed39368087

2024

PD-L1 restrains PD-1Nrp1 Treg cells to suppress inflammation-driven colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dakota B Poschel,John D Klement,Alyssa D Merting,Chunwan Lu,Yang Zhao,Dafeng Yang,Wei Xiao,Huabin Zhu,Ponnala Rajeshwari,Michael Toscano,Kimya Jones,Amanda Barrett,Roni J Bollag,Padraic G Fallon,Huidong Shi,Kebin Liu

Cell genomics 4:100659 PubMed39317187

2024

CXCR4 orchestrates the TOX-programmed exhausted phenotype of CD8 T cells via JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Canhui Cao,Miaochun Xu,Ye Wei,Ting Peng,Shitong Lin,Xiaojie Liu,Yashi Xu,Tian Chu,Shiyi Liu,Ping Wu,Bai Hu,Wencheng Ding,Li Li,Ding Ma,Peng Wu

Cell communication and signaling : CCS 22:429 PubMed39227807

2024

NDR1 mediates PD-L1 deubiquitination to promote prostate cancer immune escape via USP10.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Fu,Jinxin Li,Zuodong Xuan,Zeyuan Zheng,Yankuo Liu,Zeyi Zhang,Jianzhong Zheng,Min Zhong,Bin Liu,Yifan Du,Lei Zhang,Huimin Sun

Human gene therapy 33:649-663 PubMed35272497

2022

Bispecific Antibody Expressed by an Oncolytic Herpes Simplex Virus Type 2 Can Transform Heterologous T Cells Into Uniform Tumor Killer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Jin,Runyang Wang,Junhan Yang,Han Hu,Di Wang,Linkang Cai,Zhizheng Fang,Shuang Dong,Sheng Hu,Yang Wang,Binlei Liu

Cancer communications (London, England) 42:17-36 PubMed34981670

2022

Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Cheng,Kaikai Ding,Pengxiang Chen,Jianxiong Ji,Tao Luo,Xiaofan Guo,Wei Qiu,Chunhong Ma,Xue Meng,Jian Wang,Jinming Yu,Yuan Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-2 Receptor alpha antibody [EPR22588-18]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise