Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples.
Images
![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB264557), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-epr22816-65-ab264557--flow-cytometry-img67778.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB264557)")
![Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB264557), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-epr22816-65-ab264557--western-blot-img36632.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB264557)")
![Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB264557), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-epr22816-65-ab264557--western-blot-img7046.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (AB264557)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Alternative names
CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA
Recommended products
Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22816-65
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-epr22816-65-ab264557--flow-cytometry-img67778.jpg "Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)")
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
Flow cytometric analysis of Mouse splenocytes cells labelling IL-2 Receptor alpha with ab264557 at 1/500 (Right) as compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab264557 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647.
Gated on viable cells.
![Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-epr22816-65-ab264557--western-blot-img36632.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)")
Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 5 seconds.
This recombinant protein with LIF-tag (LIF tag MW is ~40KD) expressed from a mammalian - HEK-293 expression system.
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution
All lanes: His-tagged mouse IL-2 Receptor alpha recombinant protein (aa22-236) 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
![Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-epr22816-65-ab264557--western-blot-img7046.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)")
Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 3 minutes; Lane 2: 81 seconds.
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution
Lane 1: Mouse thymus lysate at 20 µg
Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 50 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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