AB8235

Anti-IL-2 Receptor alpha antibody [MEM-181]

Name: Anti-IL-2 Receptor alpha antibody [MEM-181] (ab8235) | Abcam
Brand: Abcam Limited
SKU: AB8235
Rating: 5 (1 reviews)

(1 Review)

(7 Publications )

Mouse Monoclonal IL2RA antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing IL2RA protein.

2 Images

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Flow Cyt

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Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Flow cytometry analysis showing separation of human PHA stimulated peripheral blood mononuclear cells stained using ab8235 (concentration in sample 2 µg/ml, GAM APC, red-filled) from human PHA stimulated peripheral blood mononuclear cells unstained by primary antibody (GAM APC, black-dashed).

660 APC

APC Anti-IL-2 Receptor alpha antibody [MEM-181]
785 APC/Cy7®

APC/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181]
723 Alexa Fluor® 700

Alexa Fluor® 700 Anti-IL-2 Receptor alpha antibody [MEM-181]
519 FITC

FITC Anti-IL-2 Receptor alpha antibody [MEM-181]
578 PE

PE Anti-IL-2 Receptor alpha antibody [MEM-181]
778 PE/Cy7®

PE/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-IL-2 Receptor alpha antibody [MEM-181]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-181

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing IL2RA protein. The exact immunogen used to generate this antibody is proprietary information.

P01589

Epitope

extracellular

Specificity

CD25 antigen (human).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information IL2RA

Alternative Names

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:1951-1964 PubMed39963418

2025

Functionalized Carbon Dots With Intrinsic Wnt/-Catenin Inhibition to Synergistically Promote 5-Fluorouracil Chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Yang,Mingyue Zhou,Tianpeng Yin,Cai-Yun Wang,Guo-Yuan Zhu,Li-Ping Bai,Zhi-Hong Jiang,Wei Zhang

Molecular brain 15:18 PubMed35183222

2022

The adaptor protein PICK1 targets the sorting receptor SorLA.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Binkle,Marcel Klein,Uwe Borgmeyer,Dietmar Kuhl,Guido Hermey

Environmental toxicology and pharmacology 80:103434 PubMed32544427

2020

Combined effects of BPA and PFOS on fetal cardiac development: In vitro and in vivo experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Zhou,Wei Cheng,Yan Feng,Wei Wang,Fan Liang,Fei Luo,Shoufei Yang,Yan Wang

Cancer science 110:2507-2519 PubMed31215741

2019

Interaction of transforming growth factor-β-Smads/microRNA-362-3p/CD82 mediated by M2 macrophages promotes the process of epithelial-mesenchymal transition in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghui Zhang,Feng Huang,Yongliang Yao,Jianjun Wang,Jue Wei,Qiong Wu,Shihao Xiang,Ling Xu

The Journal of comparative neurology 522:3386-402 PubMed24715575

2014

Spatiotemporal expression analysis of the growth factor receptor SorCS3.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Oetjen,Claudia Mahlke,Irm Hermans-Borgmeyer,Guido Hermey

PloS one 8:e62435 PubMed23626818

2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

Applications

ICC/IF

Species

Human

Juan L Brusés

Traffic (Copenhagen, Denmark) 9:980-94 PubMed18315530

2008

Different motifs regulate trafficking of SorCS1 isoforms.

Applications

ICC/IF

Species

Mouse

Morten S Nielsen,Sady J Keat,Jida W Hamati,Peder Madsen,Jakob J Gutzmann,Arne Engelsberg,Karen M Pedersen,Camilla Gustafsen,Anders Nykjaer,Jørgen Gliemann,Irm Hermans-Borgmeyer,Dietmar Kuhl,Claus M Petersen,Guido Hermey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-2 Receptor alpha antibody [MEM-181]

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (7)

Product promise