Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Transfected cell line - Human samples. Cited in 6 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101192.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--immunocytochemistry-immunofluorescence-img42077.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441)")
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--western-blot-img4762.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441)")
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--western-blot-img161687.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441)")
Publications
Filter by
- Frontiers in immunology 13:10171322022Cysteine-specific Zr-labeled anti-CD25 IgG allows immuno-PET imaging of interleukin-2 receptor-α on T cell lymphomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Hee Lee,Kyung-Ho Jung,Mina Kim,Kyung-Han LeePubMed 36591250
- Frontiers in immunology 13:8717422022The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Tian,Huan Gao,Yingying Ma,Lizhe Zhu,Yan Zhou,Yanwei Shen,Bo WangPubMed 36159822
- Frontiers in immunology 13:8595812022Immunity and Extracellular Matrix Characteristics of Breast Cancer Subtypes Based on Identification by T Helper Cells Profiling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhou,Qi Tian,Huan Gao,Lizhe Zhu,Ying Zhang,Chenchen Zhang,Jiao Yang,Bo WangPubMed 35795662
- Cell death & disease 13:1602022HOXA-AS2 contributes to regulatory T cell proliferation and immune tolerance in glioma through the miR-302a/KDM2A/JAG1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanhong Zhong,Bei Tao,Xianglong Li,Wei Xiang,Lilei Peng,Tangming Peng,Ligang Chen,Xiangguo Xia,Jian You,Xiaobo YangPubMed 35181676
- Annals of translational medicine 9:14102021miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Cao,Yunchuan Li,Yong Li,Lan LiPubMed 34733962
- Veterinary pathology 57:160-1712019Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeather Tillman,Laura J Janke,Amy Funk,Peter Vogel,Jerold E RehgPubMed 31736441
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Transfected cell line - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes - |
Species Human | Dilution info 1/200 | Notes - |
Species Transfected cell line - Human | Dilution info 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Alternative names
Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA, CD25
Recommended products
Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Transfected cell line - Human samples. Cited in 6 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
SP176
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img101192.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Formalin-fixed, paraffin-embedded human endometrium tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--immunocytochemistry-immunofluorescence-img42077.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified ab231441 at 1/50 (9.5μg/mL). Cells were fixed in 100% methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--western-blot-img4762.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/mL
All lanes: WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 31 kDa
Observed band size: 50 kDa
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--western-blot-img161687.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/200 dilution
All lanes: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 31 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110174.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--western-blot-img440765.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Blocking and diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/1000 dilution
Lane 1: HEK-293T transfected with an empty vector whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with a human IL2RA fragment (aa22-272) expression vector containing a His tag whole cell lysate at 20 µg
Lane 3: HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 50 kDa
Exposure time: 5s
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-ab231441--western-blot-img440765.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com