Rabbit Recombinant Monoclonal IL2RA antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Transfected cell line - Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (AB238272), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-bsa-and-azide-free-ab238272--immunocytochemistry-immunofluorescence-img42077.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (AB238272)")
![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (AB238272), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-bsa-and-azide-free-ab238272--western-blot-img440765.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (AB238272)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Transfected cell line - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Transfected cell line - Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with Anti-IL-2 Receptor alpha antibody [SP176] ab231441 for 10 minutes at room temperature. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Alternative names
CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA
Recommended products
Rabbit Recombinant Monoclonal IL2RA antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP176
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238272 is the carrier-free version of Anti-IL-2 Receptor alpha antibody [SP176] ab231441.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (ab238272), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-bsa-and-azide-free-ab238272--immunocytochemistry-immunofluorescence-img42077.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (ab238272)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (ab238272)
Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified Anti-IL-2 Receptor alpha antibody [SP176] ab231441 at 1/50 (9.5μg/mL). Cells were fixed in 100% methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-2 Receptor alpha antibody [SP176] ab231441).![Western blot - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (ab238272), expandable thumbnail](https://content.abcam.com/products/images/il-2-receptor-alpha-antibody-sp176-bsa-and-azide-free-ab238272--western-blot-img440765.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (ab238272)")
Western blot - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (ab238272)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL-2 Receptor alpha antibody [SP176] ab231441)
Blocking and diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
All lanes: Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (Anti-IL-2 Receptor alpha antibody [SP176] ab231441) at 1/1000 dilution
Lane 1: HEK-293T transfected with an empty vector whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with a human IL2RA fragment (aa22-272) expression vector containing a His tag whole cell lysate at 20 µg
Lane 3: HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 50 kDa
Exposure time: 5s
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