Anti-IL-2 Receptor alpha antibody [YTH906.9HL] - BSA and Azide free
- Recombinant
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Mouse Recombinant Monoclonal IL2RA antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.
View Alternative Names
CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA
- Flow Cyt
Lab
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [YTH906.9HL] - BSA and Azide free (AB283595)
This data was developed using ab283576 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-2 Receptor alpha with ab283576 at 1/1000 dilution (0.1ug) (Right) compared with a Rat monoclonal IgG (Left) isotype control. Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG or ab283576. Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Gated on viable lymphocytes.
- Flow Cyt
Lab
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [YTH906.9HL] - BSA and Azide free (AB283595)
This data was developed using ab283576 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HDLM2 (Human Hodgkin lymphoma, Right) cells labelling IL-2 Receptor alpha with ab283576 at 1/1000 dilution (0.1ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 25894527). Gated on viable cells.
Related conjugates and formulations (1)
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Anti-IL-2 Receptor alpha antibody [YTH906.9HL]
Reactivity data
Product details
ab283595 is the carrier-free version of ab283576.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com