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AB5978

Anti-IL-21 antibody

4

(2 Reviews)

|

(13 Publications)

Rabbit Polyclonal IL-21 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human IL21 aa 100-150.

View Alternative Names

Interleukin-21, IL-21, Za11, IL21

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (AB5978)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (AB5978)

ab5978 at 2ug/ml staining of IL21 in K562 cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21 antibody (AB5978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21 antibody (AB5978)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-21 antibody (ab5978) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21 antibody (AB5978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21 antibody (AB5978)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL-21 antibody (ab5978) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western blot - Anti-IL-21 antibody (AB5978)
  • WB

Supplier Data

Western blot - Anti-IL-21 antibody (AB5978)

1h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-21 antibody (ab5978) at 1 µg/mL

Lane 1:

Human tonsil tissue lysate at 15 µg

Lane 2:

Mouse spleen tissue lysate at 15 µg

Lane 3:

Rat spleen tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 18 kDa

false

Western blot - Anti-IL-21 antibody (AB5978)
  • WB

Unknown

Western blot - Anti-IL-21 antibody (AB5978)

All lanes:

Western blot - Anti-IL-21 antibody (ab5978) at 1 µg/mL

All lanes:

Western blot - Recombinant human IL-21 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-il-21-protein-ab73250'>ab73250</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 17 kDa

true

Exposure time: 8min

Western blot - Anti-IL-21 antibody (AB5978)
  • WB

Supplier Data

Western blot - Anti-IL-21 antibody (AB5978)

1h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-21 antibody (ab5978) at 0.5 µg/mL

All lanes:

Human IL-21 recombinant protein at 30 ng

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human IL21 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9HBE4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (PubMed : 11081504, PubMed : 15178704). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity).
See full target information IL21
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scandinavian journal of immunology 100:e13401 PubMed39155774

2024

NrCAM activates the NF-κB signalling pathway by competitively binding to SUMO-1 and promotes Th17 cell differentiation in Graves' disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjiao Huang,Lijuan Zhang,Yingying Zhou,Shuiying Zhao,Jiao Wang

Journal of veterinary science 23:e2 PubMed34931503

2021

Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and co-infection in the lungs of piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Jing Wang,Xiong Zhang,Chunping Zhao,Sixuan Zhou,Chunlin Du,Ya Tan,Yu Zhang,Kaizhi Shi

Cancers 13: PubMed34359579

2021

Follicular Helper T Cells Remodel the Immune Microenvironment of Pancreatic Cancer via Secreting CXCL13 and IL-21.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Lin,Longyun Ye,Xu Wang,Zhenyu Liao,Jia Dong,Ying Yang,Rulin Zhang,Hao Li,Pengcheng Li,Lei Ding,Tianjiao Li,Wuhu Zhang,Shuaishuai Xu,Xuan Han,Huaxiang Xu,Wenquan Wang,Heli Gao,Xianjun Yu,Liang Liu

Molecular therapy oncolytics 20:71-81 PubMed33575472

2021

A novel vaccinia virus enhances anti-tumor efficacy and promotes a long-term anti-tumor response in a murine model of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wang,Jiwei Wang,Zhe Zhang,Hua Cao,Wenli Yan,Yongchao Chu,Louisa S Chard Dunmall,Yaohe Wang

Immunopharmacology and immunotoxicology 42:632-642 PubMed33081532

2020

Effects of treatment with hydroxychloroquine on the modulation of Th17/Treg ratio and pregnancy outcomes in women with recurrent implantation failure: clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Sadeghpour,Morteza Ghasemnejad Berenji,Hamid Nazarian,Tohid Ghasemnejad,Mohammad Hadi Nematollahi,Sina Abroon,Shahrokh Paktinat,Heidar Heidari Khoei,Hojjat Ghasemnejad Berenji,Marefat Ghaffari Novin

International journal of medical sciences 17:3065-3072 PubMed33173427

2020

IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zongtao Liu,Yixuan Wang,Jiawei Shi,Si Chen,Li Xu,Fei Li,Nianguo Dong

Theranostics 10:4250-4264 PubMed32226551

2020

Bcl-6-directed follicular helper T cells promote vascular inflammatory injury in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Ziqi Yang,Peilong Lai,Zijing Huang,Xiaowei Sun,Tian Zhou,Chang He,Xialin Liu

Life sciences 245:117390 PubMed32007574

2020

Circulating follicular T helper cells and humoral reactivity in rheumatic heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zongtao Liu,Yixuan Wang,Fei Li,Fei Xie,Ming Liu,Jiawei Shi,Nianguo Dong

International journal of molecular medicine 44:1697-1706 PubMed31573051

2019

IL‑21/IL‑21R inhibit tumor growth and invasion in non‑small cell lung cancer cells via suppressing Wnt/β‑catenin signaling and PD‑L1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Xue,Ping Yang,Qiongying Wei,Xiaoping Li,Lan Lin,Tingyan Lin

Chinese medical journal 130:2101-2106 PubMed28836555

2017

Upregulation of Interleukin 21 and Interleukin 21 Receptor in Patients with Dermatomyositis and Polymyositis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Ying Hou,Ting-Jun Dai,Chuan-Zhu Yan
View all publications
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