Anti-IL-22RA1 antibody [EPR28682-24]

• WB

Supplier Data

Western blot - Anti-IL-22RA1 antibody [EPR28682-24] (AB324611)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : TF-1, Jurkat (PMID : 15308104), Caco-2, cerebellum (PMID : 15308104).

Samples are non-boiled as boiling may cause protein aggregation.

In lane 5-12, the lysate was stored at -80℃ prior to Western blotting.

The bands beneath the target band (75 kDa) are likely to be degradation products or non-specific bands.

In lanes 1-4, to minimize protein degradation, tissues were lysed immediately after harvest and used for Western blotting as soon as possible.

The blot of lane 11-12 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

Lanes 1-10 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000, lanes 11-12 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL-22RA1 antibody [EPR28682-24] (ab324611) at 1/1000 dilution

Lane 1:

AsPC-1 (human Pancreas epithelial cell) whole cell fresh lysate at 48 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell fresh lysate at 48 µg

Lane 3:

TF-1 (human erythroleukemia erythroblast) whole cell fresh lysate at 48 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell fresh lysate at 48 µg

Lane 5:

AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 48 µg

Lane 6:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 7:

TF-1 (human erythroleukemia erythroblast) whole cell lysate at 48 µg

Lane 8:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 48 µg

Lane 9:

SW1116 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 10:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 11:

Human colon tissue lysate at 48 µg

Lane 12:

Human cerebellum tissue lysate at 48 µg

Secondary

Lanes 1 - 10:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 11 - 12:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 180s