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AB222104

Anti-IL-23R antibody [EPR22838-4]

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(2 Publications)

Rabbit Recombinant Monoclonal IL-23R antibody. Suitable for Flow Cyt (Intra), I-ELISA, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 2 publications.

View Alternative Names

Interleukin-23 receptor, IL-23 receptor, IL-23R, IL23R

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-23R antibody [EPR22838-4] (AB222104)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-23R antibody [EPR22838-4] (AB222104)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-23R with ab222104 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged IL-23R expression vector is observed. Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-IL-23R antibody [EPR22838-4] (AB222104)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-23R antibody [EPR22838-4] (AB222104)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with myc-tagged IL-23R expression vector cells labeling IL-23R with ab222104 at 1/50 dilution (1μg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG (black) or ab225248 (red). Then fixed with 2% PFA followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647. Gated on myc(-)(Left) and myc(+)(Right) population respectively.

Indirect ELISA - Anti-IL-23R antibody [EPR22838-4] (AB222104)
  • I-ELISA

Unknown

Indirect ELISA - Anti-IL-23R antibody [EPR22838-4] (AB222104)

ELISA analysis of Human IL23R recombinant protein at 1000 ng/mL with ab222104. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22838-4

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.
See full target information IL23R
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed41008831

2025

Proteomic Identification of IL4I1 as a Therapeutic Target in P53-Mutant Endometrial Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Li,Ruonan Zhang,Tiantian Sui,Kai Wang,Yiran Li,Xuezhen Luo,Qizhi He

Journal of clinical immunology 42:1638-1652 PubMed35829840

2022

A Novel Homozygous Stop Mutation in IL23R Causes Mendelian Susceptibility to Mycobacterial Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Frederik Staels,Flaminia Lorenzetti,Kerstin De Keukeleere,Mathijs Willemsen,Margaux Gerbaux,Julika Neumann,Thomas Tousseyn,Emanuela Pasciuto,Paul De Munter,Xavier Bossuyt,Rik Gijsbers,Adrian Liston,Stephanie Humblet-Baron,Rik Schrijvers
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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