Anti-IL-24 antibody

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-24 antibody (AB115207)

Fow cytometry analysis of IL24 in Jurkat cells using ab115207at 5μg/10⁶ cells. The shaded histogram represents cells only, green represents isotype control, and pink represents anti-IL24 antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-24 antibody (AB115207)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, staining IL24 with ab114207 at 10 µg/ml.

• WB

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Western blot - Anti-IL-24 antibody (AB115207)

All lanes:

Western blot - Anti-IL-24 antibody (ab115207) at 0.5 µg/mL

Lane 1:

Jurkat cell lysate

Lane 2:

Jurkat cell lysate with IL24 blocking peptide

Predicted band size: 23 kDa

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• WB

CiteAb

Western blot - Anti-IL-24 antibody (AB115207)

IL-24 western blot using anti-IL-24 antibody ab115207. Publication image and figure legend from Margaryan, N. V., Gilgur, A., et al., 2016, Int J Mol Sci, PubMed 27011171.

ab115207 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab115207 please see the product overview.

Effect of treatment of C8161 melanoma cells with HeMn melanocyte conditioned medium (HeMn-CM). (A) C8161 cells treated with HeMn-CM show morphologic changes such as dendritic shape with long cytoplasmic extensions reminiscent of melanocyte differentiation. (40× original magnification); (B) Although, Melanoma Differentiation Associated protein 7 (MDA-7) was detected in C8161 cells treated with HeMn-CM, the melanin synthesis associated enzyme tyrosinase was not; (C) C8161 cells treated with HeMn-CM showed similar proliferation rate and cell viability as non-treated control C8161 cells making potential toxic effect of HeMn-CM on C8161 unlikely; (D) Western blot analysis show that HeMn-CM treated C8161 express lower levels of Nodal, P-SMAD2 and P-ERK1/2 compared to untreated control C8161 cells. Numbers below WB bands represent densitometric units, normalized to actin loading control, total SMAD2/3 or total ERK1/2, as appropriate, relative to control.

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