Anti-IL-24 antibody [EPR28683-20]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-24 antibody [EPR28683-20] (AB325236)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human IL-24 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling IL-24 with ab325236 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-24 antibody [EPR28683-20] (AB325236)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A-673 (human muscle Ewings sarcoma cell line) treated with 300ng/ml BFA for 24 hours (Left) A375 (human malignant melanoma epithelial cell) treated with 300ng/ml BFA for 24 hours (Right) cells labelling IL-24 with ab325236 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : A-673 treated with 300ng/ml BFA for 24 hours

• WB

Lab

Western blot - Anti-IL-24 antibody [EPR28683-20] (AB325236)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : A-673, A-673 treated with 300ng/ml BFA for 24 hours

The identity of the higher MW band at approximately 75 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-24 antibody [EPR28683-20] (ab325236) at 1/1000 dilution

Lane 1:

Untreated A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A375 treated with 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg

Lane 3:

Untreated A-673 (human muscle Ewings sarcoma cell line) whole cell lysate at 20 µg

Lane 4:

A-673 treated with 300 ng/ml BFA for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 23 kDa,36 kDa

false

Exposure time: 114s

• WB

Lab

Western blot - Anti-IL-24 antibody [EPR28683-20] (AB325236)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 75 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-IL-24 antibody [EPR28683-20] (ab325236) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human IL24 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa

false

Exposure time: 1s