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AB250239

Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL-25 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human samples.

View Alternative Names

IL17E, UNQ3120/PRO10272, IL25, Interleukin-25, IL-25, Interleukin-17E, IL-17E

1 Images
Western blot - Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free (AB250239)
  • WB

Supplier Data

Western blot - Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free (AB250239)

This data was developed using ab180594, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IL-25 antibody [EPR5743(2)] (<a href='/en-us/products/primary-antibodies/il-25-antibody-epr57432-ab180594'>ab180594</a>) at 1/5000 dilution

All lanes:

Human IL-17E recombinant protein aa1-145 at 0.05 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5743(2)

Isotype

IgG

Carrier free

Yes

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250239 is the carrier-free version of ab180594.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production (PubMed : 15860795, PubMed : 25821217). Promotes and augments T-helper type 2 responses locally or systemically (PubMed : 25821217). Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction (By similarity). In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13 (PubMed : 25821217). Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways (PubMed : 15860795). Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13 (PubMed : 11754819).
See full target information IL25
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com