AB252216

Anti-IL-27-A antibody [EPR18247-86]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal IL-27-A antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

IL27A, IL30, IL27, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, Interleukin-30, p28

5 Images

Flow Cytometry (Intracellular) - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast cell line) cells labeling IL-27-A with ab252216 at 1/100 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling IL-27-A with ab252216 at 1/100 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Unknown

Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

IL-27-A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab252216 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab252216 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).
Lane 2 : ab252216 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab252216 in HeLa whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 1 minute.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19620301, PMID : 12121660).

All lanes:

Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-86] (ab252216)

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Supplier Data

Western blot - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 12121660, PMID : 21385171).

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure times : Lanes 1-3, 5 : 3 minutes; Lane 4 : 60 seconds.

All lanes:

Western blot - Anti-IL-27-A antibody [EPR18247-86] (ab252216) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 4:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 5:

Rat spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa,48 kDa

false

Supplier Data

Western blot - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 12121660, PMID : 21385171)

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-IL-27-A antibody [EPR18247-86] (ab252216) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage cell line) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 treated with 100ng/ml LPS for 6h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa,48 kDa

false

Exposure time: 3min

Carrier free

Anti-IL-27-A antibody [EPR18247-86] - BSA and Azide free (Detector)
Carrier free

Anti-IL-27-A antibody [EPR18247-86] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18247-86

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of pro-inflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 also has an effect on cytokine production, it suppresses pro-inflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

See full target information IL27

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:125 PubMed40794120

2025

NAT10 Mediates Cardiac Fibrosis Induced by Myocardial Infarction Through ac4C Modification of TGFBR1 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Zhou,Yu Chen,Jinfa Chen,Guojin Xia,Junyi Zeng,Liang Wang

Cardiovascular diagnosis and therapy 11:422-434 PubMed33968620

2021

The interplay between HIF-1α and long noncoding GAS5 regulates the JAK1/STAT3 signalling pathway in hypoxia-induced injury in myocardial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanwei Li,Bing Song,Jinlei Liu,Yuqiang Li,Jiebing Wang,Na Liu,Wei Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-27-A antibody [EPR18247-86]

Flow Cytometry (Intracellular) - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Flow Cytometry (Intracellular) - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Western blot - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Western blot - Anti-IL-27-A antibody [EPR18247-86] (AB252216)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise