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AB126713

Anti-IL-28A + IL-28B antibody [EPR5328(2)]

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Rabbit Recombinant Monoclonal IFNL2 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples.

View Alternative Names

IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

2 Images
Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (AB126713)
  • WB

Lab

Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (AB126713)

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/5000 dilution

All lanes:

Human IL-28A (amino acids 27-200) recombinant protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 19 kDa

false

Exposure time: 120s

Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (AB126713)
  • WB

Unknown

Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (AB126713)

Exposure time

Lane 1 : 40 seconds

Lane 2 : 3 second

Buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/1000 dilution

Lane 1:

Human IL-28A recombinant protein (aa 27 to 200) at 0.01 µg

Lane 2:

House made His-tagged full length human IL-28B recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • Carrier free

    Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5328(2)

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL2

Additional targets

IFNL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com