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Rabbit Recombinant Monoclonal IFNL2 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human samples.

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Images

Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (AB248141), expandable thumbnail
  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (AB248141), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Predicted
Recombinant full length protein - Human
Tested

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Target data

Function

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Targets

IFNL3

Alternative names

Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A, IL28A, ZCYTO20, IFNL2

Recommended products

Rabbit Recombinant Monoclonal IFNL2 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human samples.

Alternative names

Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A, IL28A, ZCYTO20, IFNL2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR5328(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248141 is the carrier-free version of Anti-IL-28A + IL-28B antibody [EPR5328(2)] ab126713.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141), expandable thumbnail

    Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)

    Exposure time

    Lane 1: 40 seconds

    Lane 2: 3 second

    Buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (Anti-IL-28A + IL-28B antibody [EPR5328(2)] ab126713) at 1/1000 dilution

    Lane 1: Human IL-28A recombinant protein (aa 27 to 200) at 0.01 µg

    Lane 2: House made His-tagged full length human IL-28B recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141), expandable thumbnail

    Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)

    This data was developed using Anti-IL-28A + IL-28B antibody [EPR5328(2)] ab126713, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] (Anti-IL-28A + IL-28B antibody [EPR5328(2)] ab126713) at 1/5000 dilution

    All lanes: Human IL-28A (amino acids 27-200) recombinant protein

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 19 kDa

    Exposure time: 120s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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