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Rabbit Recombinant Monoclonal IL-2RG antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (AB282585), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (AB282585), expandable thumbnail
  • Immunoprecipitation - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (AB282585), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (AB282585), expandable thumbnail
  • Western blot - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (AB282585), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Expected
Not recommended
Tested
Not recommended
Rat
Not recommended
Expected
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Common subunit for the receptors for a variety of interleukins. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed:15123770).

Alternative names

Cytokine receptor common subunit gamma, Interleukin-2 receptor subunit gamma, gammaC, p64, IL-2 receptor subunit gamma, IL-2R subunit gamma, IL-2RG, IL2RG

Recommended products

Rabbit Recombinant Monoclonal IL-2RG antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Alternative names

Cytokine receptor common subunit gamma, Interleukin-2 receptor subunit gamma, gammaC, p64, IL-2 receptor subunit gamma, IL-2R subunit gamma, IL-2RG, IL2RG

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24694-133

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab282585 is the carrier-free version of Anti-IL-2RG antibody [EPR24694-133] ab273023.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585), expandable thumbnail

    Western blot - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585)

    This data was developed using 273023, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11418669 and PMID: 19329337). The 50kDa-70kDa bands correspond to the mature form and immature form.

    Negative control: HT-29 (PMID: 8530527).

    Exposure time: Lane 1: 3 minutesLane 2: 15 seconds Lanes 3-8: 48 seconds

    All lanes: Western blot - Anti-IL-2RG antibody [EPR24694-133] (Anti-IL-2RG antibody [EPR24694-133] ab273023) at 1/1000 dilution

    Lane 1: Human spleen tissue lysate at 20 µg

    Lane 2: Human lymphoma tissue lysate at 20 µg

    Lane 3: Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate at 20 µg

    Lane 4: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 5: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 7: Mouse spleen tissue lysate at 20 µg

    Lane 8: Rat spleen tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 3 - 8: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa

    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585)

    This data was developed using Anti-IL-2RG antibody [EPR24694-133] ab273023, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IL-2RG with Anti-IL-2RG antibody [EPR24694-133] ab273023 at 1/500 (1.126 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution. Positive staining in lamina propria of human colon. The section was incubated with Anti-IL-2RG antibody [EPR24694-133] ab273023 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585), expandable thumbnail

    Immunoprecipitation - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585)

    This data was developed using Anti-IL-2RG antibody [EPR24694-133] ab273023, the same antibody clone in a different buffer formulation.

    IL-2RG was immunoprecipitated from 0.35 mg Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate 10ug with Anti-IL-2RG antibody [EPR24694-133] ab273023 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IL-2RG antibody [EPR24694-133] ab273023 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate 10ug

    Lane 2: Anti-IL-2RG antibody [EPR24694-133] ab273023 IP in Daudi whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL-2RG antibody [EPR24694-133] ab273023 in Daudi whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds

    All lanes: Immunoprecipitation - Anti-IL-2RG antibody [EPR24694-133] (Anti-IL-2RG antibody [EPR24694-133] ab273023)

    Predicted band size: 42 kDa

    Observed band size: 50-70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585)

    This data was developed using Anti-IL-2RG antibody [EPR24694-133] ab273023, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling IL-2RG with Anti-IL-2RG antibody [EPR24694-133] ab273023 at 1/500 (1.126 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution. Positive staining in human gastric cancer. The section was incubated with Anti-IL-2RG antibody [EPR24694-133] ab273023 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585), expandable thumbnail

    Western blot - Anti-IL-2RG antibody [EPR24694-133] - BSA and Azide free (ab282585)

    This data was developed using the same antibody clone in a different buffer formulation (abAB273023).

    Western blot: Anti-IL2RG antibody [EPR24694-133] (Anti-IL-2RG antibody [EPR24694-133] ab273023) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-IL-2RG antibody [EPR24694-133] ab273023 was shown to bind specifically to IL2RG. A band was observed at 55 kDa in wild-type K562 cell lysates with no signal observed at this size in IL2RG knockout cell line. To generate this image, wild-type and IL2RG knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-IL-2RG antibody [EPR24694-133] (Anti-IL-2RG antibody [EPR24694-133] ab273023) at 1/1000 dilution

    Lane 1: Wild-type K562 cell lysate at 20 µg

    Lane 2: IL2RG knockout K562 cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 55 kDa

    Western blot: Anti-IL2RG antibody [EPR24694-133] (Anti-IL-2RG antibody [EPR24694-133] ab273023) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-IL-2RG antibody [EPR24694-133] ab273023 was shown to bind specifically to IL2RG. A band was observed at 55 kDa in wild-type K562 cell lysates with no signal observed at this size in IL2RG knockout cell line. To generate this image, wild-type and IL2RG knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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