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AB190941

Anti-IL-3 antibody

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(1 Publication)

Rabbit Polyclonal IL-3 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IL3 aa 100-150.

View Alternative Names

Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-3 antibody (AB190941)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-3 antibody (AB190941)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat spleen tissue labeling IL3 with ab190941 at 2 μg/ml. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL3 Antibody ab190941, overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit, with DAB as the chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human IL3 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P08700

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 2556442). Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated (PubMed : 10779277, PubMed : 32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed : 23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed : 29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed : 27862234).
See full target information IL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:2032 PubMed32341344

2020

Immunoglobulin expression and the humoral immune response is regulated by the non-canonical poly(A) polymerase TENT5C.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Bilska,Monika Kusio-Kobiałka,Paweł S Krawczyk,Olga Gewartowska,Bartosz Tarkowski,Kamil Kobyłecki,Dominika Nowis,Jakub Golab,Jakub Gruchota,Ewa Borsuk,Andrzej Dziembowski,Seweryn Mroczek
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Product promise

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For full details, please see our Terms & Conditions

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