AB167159

Anti-IL-3 antibody [EPR7964]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal IL-3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159)

Immunocytochemical analysis of Jurkat cells labeling IL3 with ab167159 at a 1/100 dilution.

Unknown

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

All lanes:

Western blot - Anti-IL-3 antibody [EPR7964] (ab167159) at 1/1000 dilution

All lanes:

Human IL3 recombinant protein (10 ng)

Predicted band size: 17 kDa

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Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

Blocking/diluting Buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-IL-3 antibody [EPR7964] (ab167159) at 1.23 µg/mL

All lanes:

Mouse IL-3 recombinant protein at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Carrier free

Anti-IL-3 antibody [EPR7964] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7964

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

WB is only recommended to detect recombinant protein, not endogenous IL-3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 2556442). Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated (PubMed : 10779277, PubMed : 32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed : 23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed : 29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed : 27862234).

See full target information IL3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2405364 PubMed39225429

2024

Nanotherapeutic Approaches of Interleukin-3 to Clear the α-Synuclein Pathology in Mouse Models of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlong Zhang,Jian Ren,Liuyan Ding,Shaohui Zheng,Runfang Ma,Mengran Zhang,Yan Liu,Ruijing Liang,Yunlong Zhang

Oncology letters 17:1523-1530 PubMed30675208

2019

Evidence for a role of geranylgeranylation in renal angiomyolipoma and renal epithelioid angiomyolipoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Zhao,Jun Yuan,Qi Cheng,Ya-Ling Qi,Ke Lu,Shan-Shan Lai,Qian Sun,Yue Zhao,Lei Fang,Mei-Ling Jin,De-Cai Yu,Yu-Dong Qiu,Chao-Jun Li,Jun Chen,Bin Xue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-3 antibody [EPR7964]

Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159)

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise