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Rabbit Recombinant Monoclonal IL-3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-IL-3 antibody [EPR7964] (AB167159), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (AB167159), expandable thumbnail
  • Western blot - Anti-IL-3 antibody [EPR7964] (AB167159), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Tested
Not recommended
Expected

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed:2556442). Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated (PubMed:10779277, PubMed:32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed:23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed:12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed:29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed:27862234).

Alternative names

Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3

Recommended products

Rabbit Recombinant Monoclonal IL-3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7964
Purity
Tissue culture supernatant
Specificity

WB is only recommended to detect recombinant protein, not endogenous IL-3.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-IL-3 antibody [EPR7964] (ab167159), expandable thumbnail

    Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)

    Blocking/diluting Buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-IL-3 antibody [EPR7964] (ab167159) at 1.23 µg/mL

    All lanes: Mouse IL-3 recombinant protein at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (ab167159), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (ab167159)

    Immunocytochemical analysis of Jurkat cells labeling IL3 with ab167159 at a 1/100 dilution.

  • Western blot - Anti-IL-3 antibody [EPR7964] (ab167159), expandable thumbnail

    Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)

    All lanes: Western blot - Anti-IL-3 antibody [EPR7964] (ab167159) at 1/1000 dilution

    All lanes: Human IL3 recombinant protein (10 ng)

    Predicted band size: 17 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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