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AB37158

Anti-IL-32 antibody

5

(1 Review)

|

(17 Publications)

Rabbit Polyclonal IL-32 antibody. Suitable for ICC/IF, ELISA, WB, IHC-P and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human IL32.

View Alternative Names

NK4, TAIF, IL32, Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-32 antibody (AB37158)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-32 antibody (AB37158)

Immunofluorescence of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-32 antibody (AB37158)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-32 antibody (AB37158)

IHC-P of Human spleen tissue with ab37158 at 10 µg/ml

Western blot - Anti-IL-32 antibody (AB37158)
  • WB

Unknown

Western blot - Anti-IL-32 antibody (AB37158)

Two isoforms of IL32 can be seen

Lane 1:

Western blot - Anti-IL-32 antibody (ab37158) at 5 µg/mL

Lane 2:

Western blot - Anti-IL-32 antibody (ab37158) at 10 µg/mL

All lanes:

Human spleen cell lysate at 15 µg

Predicted band size: 26 kDa

Observed band size: 12 kDa,26 kDa

false

Western blot - Anti-IL-32 antibody (AB37158)
  • WB

Supplier Data

Western blot - Anti-IL-32 antibody (AB37158)

Lane 1:

Western blot - Anti-IL-32 antibody (ab37158) at 2 µg/mL

Lane 2:

Western blot - Anti-IL-32 antibody (ab37158) at 4 µg/mL

All lanes:

Human spleen tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, ELISA

applications

Immunogen

Synthetic Peptide within Human IL32. The exact immunogen used to generate this antibody is proprietary information.

P24001

Specificity

ab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each).

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5-10 µg/mL", "WB-species-notes": "<p>All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
See full target information IL32

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:891-899 PubMed39463328

2024

Cancer-associated fibroblast-secreted exosomes promote prostate cancer cell migration and invasion by the FGL1/SOX5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingquan Kong,Xing Wang,Yu Li,Xiansheng Zhang

Experimental dermatology 32:1451-1458 PubMed37309674

2023

Induction of IL-32 in the immune response of keratinocytes to Mycobacterium marinum infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Sang,Xiaotong Xue,Zihao Mi,Zhenzhen Wang,Xueping Yu,Lele Sun,Shanshan Ma,Zhe Wang,Hong Liu,Furen Zhang

Scientific reports 13:2864 PubMed36806315

2023

Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlin Garofano,Kameron Rashid,Michael Smith,Christine Brantner,Sumanun Suwunnakorn,David Diemert,Olivia Gordon,Anelia Horvath,Sikandar Khan,Anastas Popratiloff,Johng Rhim,Alfateh Sidahmed,Sanjay B Maggirwar,Travis J O'Brien,Minoli A Perera,Norman H Lee

Journal of translational medicine 20:207 PubMed35545774

2022

IL-32 promotes the occurrence of atopic dermatitis by activating the JAK1/microRNA-155 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chang,Bin Zhou,Zhu Wei,Yongqi Luo

Cellular & molecular immunology 18:979-991 PubMed33707686

2021

Placenta-derived IL-32β activates neutrophils to promote preeclampsia development.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Liu,Qiang Li,Hailin Ding,Guangfeng Zhao,Zhiyin Wang,Chenrui Cao,Yimin Dai,Mingming Zheng,Xiangyu Zhu,Qianwen Wu,Ya Wang,Honglei Duan,Huirong Tang,Xianyan Lu,Yayi Hou,Yali Hu

Molecular medicine reports 23: PubMed33495843

2021

IL-32 exacerbates adenoid hypertrophy via activating NLRP3-mediated cell pyroptosis, which promotes inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Junmei Zhang,Xuyuan Sun,Lingling Zhong,Bei Shen

Journal of vascular research 57:236-244 PubMed32434199

2020

Altered IL-32 Signaling in Abdominal Aortic Aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Sophy Bengts,Levar Shamoun,Anne Kunath,Daniel Appelgren,Martin Welander,Martin Björck,Anders Wanhainen,Dick Wågsäter

Hepatology communications 3:1205-1220 PubMed31497742

2019

Interleukin-32 Contributes to Human Nonalcoholic Fatty Liver Disease and Insulin Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Nassim Dali-Youcef,Michel Vix,Federico Costantino,Houssein El-Saghire,Benoit Lhermitte,Cosimo Callari,Jacopo D'Agostino,Silvana Perretta,Stefan Paveliu,Monica Gualtierotti,Edith Dumeny,Marine A Oudot,Amélie Jaulin,Doulaye Dembélé,Mirjam B Zeisel,Catherine Tomasetto,Thomas F Baumert,Michel Doffoël

Gastroenterology research and practice 2018:6578273 PubMed30402092

2018

Diverse Expression of IL-32 in Diffuse and Intestinal Types of Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mladen Pavlovic,Nevena Gajovic,Milena Jurisevic,Slobodanka Mitrovic,Gordana Radosavljevic,Jelena Pantic,Nebojsa Arsenijevic,Ivan Jovanovic

International journal of health sciences 12:29-34 PubMed29896069

2018

Functional role of human interleukin-32 and nuclear transcription factor-kB in patients with psoriasis and psoriatic arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Hani A Al-Shobaili,Jalees Farhan,Uzma Zafar,Zafar Rasheed
View all publications

Product promise

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