AB169781

Anti-IL-32 antibody [EPR5067]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal IL-32 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NK4, TAIF, IL32, Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor

2 Images

Unknown

Western blot - Anti-IL-32 antibody [EPR5067] (AB169781)

All lanes:

Western blot - Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution

Lane 1:

Human lymph node lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Lane 3:

Human fetal thymus lysate at 10 µg

Lane 4:

HT29 treated with TNF alpha lysate at 10 µg

Lane 5:

HT29 lysate at 10 µg

Lane 6:

SW480 treated with INF gamma lysate at 10 µg

Lane 7:

SW480 lysate at 10 µg

Lane 8:

293T treated with TNF alpha and IFN gamma lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

false

Lab

Western blot - Anti-IL-32 antibody [EPR5067] (AB169781)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. Loading Control : GAPDH This antibody could detect different isoforms of IL-32.

All lanes:

Western blot - Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution

All lanes:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa,27 kDa

false

Exposure time: 10s

Carrier free

Anti-IL-32 antibody [EPR5067] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5067

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.

See full target information IL32

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal 38:9579-9599 PubMed30232223

2018

Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Chul Shin,Carlos Cepeda,Ana María Estrada-Sánchez,Michael S Levine,Laya Hodaei,Yun Dai,Jai Jung,Amit Ganguly,Peter Clark,Sherin U Devaskar

PLoS neglected tropical diseases 11:e0005413 PubMed28241012

2017

Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species.

Applications

Unspecified application

Species

Unspecified reactive species

Jéssica Cristina Dos Santos,Bas Heinhuis,Rodrigo Saar Gomes,Michelle S M A Damen,Fernando Real,Renato A Mortara,Samuel T Keating,Charles A Dinarello,Leo A B Joosten,Fátima Ribeiro-Dias

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-32 antibody [EPR5067]

Western blot - Anti-IL-32 antibody [EPR5067] (AB169781)

Western blot - Anti-IL-32 antibody [EPR5067] (AB169781)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (2)

Product promise