Rabbit Recombinant Monoclonal IL-32 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-IL-32 antibody [EPR5067] (AB169781), expandable thumbnail](https://content.abcam.com/products/images/il-32-antibody-epr5067-ab169781--western-blot-img12785.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-32 antibody [EPR5067] (AB169781)")
![Western blot - Anti-IL-32 antibody [EPR5067] (AB169781), expandable thumbnail](https://content.abcam.com/products/images/il-32-antibody-epr5067-ab169781--western-blot-img409180.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-32 antibody [EPR5067] (AB169781)")
Publications
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- PLoS neglected tropical diseases 11:e00054132017Cytokines and microbicidal molecules regulated by IL-32 in THP-1-derived human macrophages infected with New World Leishmania species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJéssica Cristina Dos Santos,Bas Heinhuis,Rodrigo Saar Gomes,Michelle S M A Damen,Fernando Real,Renato A Mortara,Samuel T Keating,Charles A Dinarello,Leo A B Joosten,Fátima Ribeiro-DiasPubMed 28241012
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
Alternative names
NK4, TAIF, IL32, Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor
Recommended products
Rabbit Recombinant Monoclonal IL-32 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5067
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-IL-32 antibody [EPR5067] (ab169781), expandable thumbnail](https://content.abcam.com/products/images/il-32-antibody-epr5067-ab169781--western-blot-img12785.jpg "Western blot - Anti-IL-32 antibody [EPR5067] (ab169781)")
Western blot - Anti-IL-32 antibody [EPR5067] (ab169781)
All lanes: Western blot - Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution
Lane 1: Human lymph node lysate at 10 µg
Lane 2: Human tonsil lysate at 10 µg
Lane 3: Human fetal thymus lysate at 10 µg
Lane 4: HT29 treated with TNF alpha lysate at 10 µg
Lane 5: HT29 lysate at 10 µg
Lane 6: SW480 treated with INF gamma lysate at 10 µg
Lane 7: SW480 lysate at 10 µg
Lane 8: 293T treated with TNF alpha and IFN gamma lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa
![Western blot - Anti-IL-32 antibody [EPR5067] (ab169781), expandable thumbnail](https://content.abcam.com/products/images/il-32-antibody-epr5067-ab169781--western-blot-img409180.jpg "Western blot - Anti-IL-32 antibody [EPR5067] (ab169781)")
Western blot - Anti-IL-32 antibody [EPR5067] (ab169781)
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Loading Control: GAPDH
This antibody could detect different isoforms of IL-32.All lanes: Western blot - Anti-IL-32 antibody [EPR5067] (ab169781) at 1/1000 dilution
All lanes: Human tonsil lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 17 kDa, 27 kDa
Exposure time: 10s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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