Anti-IL-32 antibody [EPR5067] - BSA and Azide free

• WB

Lab

Western blot - Anti-IL-32 antibody [EPR5067] - BSA and Azide free (AB249525)

This data was developed using ab169781, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM /TBST. Loading Control : GAPDH This antibody could detect different isoforms of IL-32.

All lanes:

Western blot - Anti-IL-32 antibody [EPR5067] (<a href='/en-us/products/primary-antibodies/il-32-antibody-epr5067-ab169781'>ab169781</a>) at 1/1000 dilution

All lanes:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa,27 kDa

false

Exposure time: 10s

• WB

Unknown

Western blot - Anti-IL-32 antibody [EPR5067] - BSA and Azide free (AB249525)

This data was developed using ab169781, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IL-32 antibody [EPR5067] (<a href='/en-us/products/primary-antibodies/il-32-antibody-epr5067-ab169781'>ab169781</a>) at 1/1000 dilution

Lane 1:

Human lymph node lysate at 10 µg

Lane 2:

Human tonsil lysate at 10 µg

Lane 3:

Human fetal thymus lysate at 10 µg

Lane 4:

HT29 treated with TNF alpha lysate at 10 µg

Lane 5:

HT29 lysate at 10 µg

Lane 6:

SW480 treated with INF gamma lysate at 10 µg

Lane 7:

SW480 lysate at 10 µg

Lane 8:

293T treated with TNF alpha and IFN gamma lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 26 kDa

false