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AB118503

Anti-IL-33 antibody

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(9 Publications)

Rabbit Polyclonal IL-33 antibody. Suitable for IHC-P, ELISA, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human IL33 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

C9orf26, IL1F11, NFHEV, IL33, Interleukin-33, IL-33, Interleukin-1 family member 11, Nuclear factor from high endothelial venules, IL-1F11, NF-HEV

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody (AB118503)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody (AB118503)

Human Uterus, Vessels (formalin-fixed, paraffin-embedded) stained with IL33 antibody ab118503 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody (AB118503)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody (AB118503)

Immunohistochemical staining of paraffin-embedded, formalin fixed, human tonsil lymphocytes with ab118503 at 5 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody (AB118503)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody (AB118503)

Negative control - immunohistochemical staining of paraffin-embedded, formalin fixed, human seminiferous tubules with ab118503 at 5 μg/mL.

Western blot - Anti-IL-33 antibody (AB118503)
  • WB

Unknown

Western blot - Anti-IL-33 antibody (AB118503)

Lane 1:

Western blot - Anti-IL-33 antibody (ab118503) at 1 µg/mL

Lane 2:

Western blot - Anti-IL-33 antibody (ab118503) at 2 µg/mL

All lanes:

Human lymph node tissue lysate

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ELISA

applications

Immunogen

Synthetic Peptide within Human IL33 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O95760

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed : 16286016, PubMed : 19841166). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (PubMed : 17853410, PubMed : 18836528). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (PubMed : 17853410, PubMed : 18836528). Acts as an enhancer of polarization of alternatively activated macrophages (PubMed : 19841166). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (PubMed : 17853410, PubMed : 18836528). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed : 21734074). This form is rapidely lost upon angiogenic or pro-inflammatory activation (PubMed : 18787100).
See full target information IL33
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular biology (Noisy-le-Grand, France) 71:36-42 PubMed39976915

2025

Notch/IL33/ST2 signaling was involved in the maintenance of intestinal epithelial barrier through regulating tight junction after LPS stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanling Zhang,Chao Xu,Fang Li,Guoqing Chen

Open life sciences 19:20220841 PubMed38585634

2024

IL-33/soluble ST2 axis is associated with radiation-induced cardiac injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokeya Yasen,Renaguli Aikebaier,Atiguli Maimaiti,Munire Mushajiang

International journal of molecular sciences 24: PubMed37895164

2023

Flavonoid-Rich Berry Extract Enhances Nrf2/HO-1 Signaling Pathway Activation and Exerts Antiulcerative Effects In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Betul Cicek,Betul Danısman,Serkan Yildirim,Neslihan Yuce,Dragana Nikitovic,Ismail Bolat,Mehmet Kuzucu,Ertuğrul Ceyran,Ebru Bardas,Kirill S Golokhvast,Aristidis Tsatsakis,Ali Taghizadehghalehjoughi

Life science alliance 4: PubMed34127548

2021

IL-13 deficiency exacerbates lung damage and impairs epithelial-derived type 2 molecules during nematode infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alistair L Chenery,Silvia Rosini,James E Parkinson,Jesuthas Ajendra,Jeremy A Herrera,Craig Lawless,Brian Hk Chan,P'ng Loke,Andrew S MacDonald,Karl E Kadler,Tara E Sutherland,Judith E Allen

European journal of heart failure 21:482-491 PubMed30675967

2019

Irregular pacing of ventricular cardiomyocytes induces pro-fibrotic signalling involving paracrine effects of transforming growth factor beta and connective tissue growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Slawik,Lucas Adrian,Mathias Hohl,Sarah Lothschütz,Ulrich Laufs,Michael Böhm

Journal of cellular and molecular medicine 23:887-897 PubMed30478965

2018

Plasma levels of soluble ST2, but not IL-33, correlate with the severity of alcoholic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zijian Sun,Binxia Chang,Ang Huang,Shuli Hao,Miaomiao Gao,Ying Sun,Ming Shi,Lei Jin,Wei Zhang,Jun Zhao,Guangju Teng,Lin Han,Hui Tian,Qingsheng Liang,Ji-Yuan Zhang,Zhengsheng Zou

Evidence-based complementary and alternative medic 2017:2489842 PubMed28751921

2017

Evaluation of Anti-Inflammatory Activities of Qingre-Qushi Recipe (QRQS) against Atopic Dermatitis: Potential Mechanism of Inhibition of IL-33/ST2 Signal Transduction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjiao Chen,Peijun Ding,Lili Yang,Xufeng He,Chunjie Gao,Guoxun Yang,Huimin Zhang

The Tohoku journal of experimental medicine 234:255-61 PubMed25400121

2014

Interleukin-33 promotes disease progression in patients with primary biliary cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Sun,Ji-Yuan Zhang,Sa Lv,Huan Wang,Man Gong,Ning Du,Honghong Liu,Ning Zhang,Jing Jing,Chao Zhou,Fan Zhang,Zongren Wang

European journal of immunology 44:2153-64 PubMed24777946

2014

Serum amyloid A induces interleukin-33 expression through an IRF7-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Sun,Ziyan Zhu,Ni Cheng,Qian Yan,Richard D Ye
View all publications
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