Rabbit Recombinant Monoclonal IL-33 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
Constituents: 100% PBS
sELISA | |
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Mouse | Expected |
Rat | Expected |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (By similarity). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (By similarity). Acts as an enhancer of polarization of alternatively activated macrophages (By similarity). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed:34644537). In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (By similarity). This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).
Interleukin-33, IL-33, Il33
Rabbit Recombinant Monoclonal IL-33 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
Constituents: 100% PBS
ab242472 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse IL-33. The recommended pair for sandwich ELISA is:
Capture: ab242472, Mouse IL-33 Capture Antibody (unconjugated)
Detector: Anti-IL-33 antibody [EPR17831-139] - BSA and Azide free (Capture/Detector) ab242723, Mouse IL-33 Detector Antibody (unconjugated)
The reference range value is 15.63-1000 pg/mL.
Or alternatively, for Monkey, the recommended pair for sandwich ELISA is:
Capture: Anti-TNF alpha antibody [EPR18565-29] - BSA and Azide free (Capture) ab242476, Monkey TNF alpha Capture Antibody (unconjugated)
Detector: Anti-TNF alpha antibody [EPR18565-33] - BSA and Azide free (Detector) ab242978, Monkey TNF alpha Detector Antibody (unconjugated)
The reference range value is 15.63 - 1000 pg/ml.
Or alternatively, for Rat, the recommended pair for sandwich ELISA is:
Capture: Anti-IL-33 antibody [EPR17831-139] - BSA and Azide free (Capture/Detector) ab242723, Rat IL-33 Capture Antibody (unconjugated)
Detector: ab242472, Rat IL-33 Detector Antibody (unconjugated)
The reference range value is 15.63 - 1000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse IL-33 ELISA Kit ab213475).
Sandwich ELISA of Mouse/Rat IL-33 Antibody Pair - BSA and Azide free ab241729 with the capture antibody ab242472 dilution at 2 µg/mL and detector antibody Anti-IL-33 antibody [EPR17831-139] - BSA and Azide free (Capture/Detector) ab242723 dilution at 0.5 µg/mL.
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