Name: Anti-IL-33 antibody [EPR17831]
SKU: ab187060
Rating: 5 (2 reviews)

Rabbit Recombinant Monoclonal IL-33 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 18 publications.

Images

Western blot - Anti-IL-33 antibody [EPR17831] (AB187060), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Mouse	Tested	Not recommended	Tested	Tested	Tested
Rat	Tested	Not recommended	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) (ab208572). Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) (ab208572). Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information Il33

Function

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:29045903). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (By similarity). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (By similarity). Acts as an enhancer of polarization of alternatively activated macrophages (By similarity). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (By similarity). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (PubMed:34644537). In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (By similarity). This form is rapidely lost upon angiogenic or pro-inflammatory activation (By similarity).

Alternative names

Interleukin-33, IL-33, Il33

Recommended products

Rabbit Recombinant Monoclonal IL-33 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 18 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17831
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)
Blocking/Dilution: 5% NFDM/TBST
Exposure: 3 minutes
IL-33 expression is induced by LPS treatment of PMA-differentiated RAW 264.7 cells (PMID 19559631; PMID 19933859).
All lanes: Western blot - Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 2: RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 33 kDa
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)
Blocking/Dilution: 5% NFDM/TBST
Exposure: 3 minutes
All lanes: Western blot - Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution
Lane 1: Rat lung tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 10 µg
Secondary
Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR17831] (ab187060)
Intracellular Flow Cytometry analysis ofRAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50nM PMA and 5μg/ml LPS for 24h (Red) / Untreated control (Green) labeling IL-33 with ab187060 at 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody. Cells were fixed with4% paraformaldehyde and permeabilised with0.1% Tween-20.Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IL33 with ab187060 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use. Nuclear staining in endothelial cells of mouse spleen is observed (PMID: 12819012). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IL33 with ab187060 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use. Nuclear staining in endothelial cells of rat spleen is observed (PMID: 12819012). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101), ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (ab187060)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling IL33 with ab187060 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing increased nuclear staining in RAW 264.7 cells treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 μg/ml Lipopolysaccharide for 24h.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR17831] (ab187060)
Flow cytometry overlay histogram showing left, Raw264.7 treated with 50nM PMA and 5μg/ml LPS for 24h and right, negative untreated Raw264.7 stained with ab187060 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab187060) (1x 106 in 100μl at 1.0μg/ml (1/2090)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)
Immunohistochemical analysis of paraffin-embedded (A) Lung tissue from wild-type C57BL/6JGpt mice (B) Lung tissue from IL-33 knockout mice labeling IL33 with ab187060 at 1/5000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Lung tissue from wild-type C57BL/6JGpt mice and no staining on (B) Lung tissue from IL-33 knockout mice.
The section was incubated with ab187060 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)
Immunohistochemical analysis of paraffin-embedded (A) Lymph node tissue from wild-type C57BL/6JGpt mice (B) Lymph node tissue from IL-33 knockout mice labeling IL33 with ab187060 at 1/5000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Lymph node tissue from wild-type C57BL/6JGpt mice and no staining on (B) Lymph node tissue from IL-33 knockout mice.
The section was incubated with ab187060 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)
Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1/200000) (36KDa)

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Lanes 1 - 3: Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)
Lanes 1 - 3: Western blot - Anti-IL-33 antibody [EPR17831] - BSA and Azide free (Anti-IL-33 antibody [EPR17831] - BSA and Azide free ab229698)
Lane 1: Wild-type mouse lung tissue lysate (male)
Lanes 2 - 3: IL-33 knockout lung tissue lysate (male)
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 15-30 kDa
Exposure time: 38s
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)
Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) (1/200000) (36KDa)

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Lanes 1 - 9: Western blot - Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution
Lanes 1 - 9: Western blot - Anti-IL-33 antibody [EPR17831] - BSA and Azide free (Anti-IL-33 antibody [EPR17831] - BSA and Azide free ab229698)
Lane 1: Wild-type mouse spleen tissue lysate (male)
Lanes 2 - 3: IL-33 knockout spleen tissue lysate (male)
Lane 4: Wild-type mouse inguinal lymph nodes tissue lysate (male)
Lanes 5 - 6: IL-33 knockout inguinal lymph nodes tissue lysate (male)
Lane 7: Wild-type mouse submandibular lymph nodes tissue lysate (male)
Lanes 8 - 9: IL-33 knockout submandibular lymph nodes tissue lysate (male)
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 15-30 kDa
Exposure time: 38s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)
Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from IL-33 knockout mice labeling IL33 with ab187060 at 1/5000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from IL-33 knockout mice.
The section was incubated with ab187060 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).