Anti-IL-33 antibody [EPR20417]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (AB207737)

Immunohistochemical analysis of paraffin embedded human kidney cancer tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelial cells of human kidney cancer (PMID : 12819012) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (AB207737)

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on high endothelial venules of human tonsil (PMID : 12819012) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (AB207737)

Immunohistochemical analysis of paraffin embedded rat lung tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on epithelium and endothelium cells of rat lung (PMID : 18836528) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (AB207737)

Immunohistochemical analysis of paraffin embedded rat spleen tissue labeling IL33 with ab207737 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelium cells of rat spleen (PMID : 12819012, PMID : 18836528) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-IL-33 antibody [EPR20417] (AB207737)

Blocking : 5% NFDM/TBST

Exposure times :

Lanes 1-3,5 : 3 minutes

Lanes 4 : 30 seconds

All lanes:

Western blot - Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

Lane 1:

Human artery at 20 µg

Lane 2:

Human lung at 20 µg

Lane 3:

Human tonsil at 20 µg

Lane 4:

Human stomach at 20 µg

Lane 5:

Human colon at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 33 kDa

false

• WB

Lab

Western blot - Anti-IL-33 antibody [EPR20417] (AB207737)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

All lanes:

Human colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 15-30 kDa

false

Exposure time: 80s

• WB

Supplier Data

Western blot - Anti-IL-33 antibody [EPR20417] (AB207737)

The level of IL33 expression can be induced by PMA and LPS treatment (PMID : 19559631).

Blocking : 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia monocyte), whole cell lysate at 10 µg

Lane 2:

THP-1 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA, <a href='/en-us/products/biochemicals/phorbol-12-myristate-13-acetate-pma-pkc-activator-ab120297'>ab120297</a>) and 5 ug/ml lipopolysaccharides (LPS) for 24 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 30 kDa

Observed band size: 33 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-IL-33 antibody [EPR20417] (AB207737)

Blocking : 5% NFDM/TBST

Exposure times :

Lane 1 : 3 seconds

Lane 2 : 10 seconds

Lane3 : 5 seconds

All lanes:

Western blot - Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

Lane 1:

Rat spleen at 10 µg

Lane 2:

Rat kidney at 10 µg

Lane 3:

Rat lung at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 30 kDa

Observed band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-IL-33 antibody [EPR20417] (AB207737)

All lanes:

Western blot - Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

All lanes:

Western blot - Recombinant Rat IL-33 protein (<a href='/en-us/products/proteins-peptides/recombinant-rat-il-33-protein-ab207126'>ab207126</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 30 kDa

Observed band size: 20 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-IL-33 antibody [EPR20417] (AB207737)

Blocking : 5% NFDM/TBST

All lanes:

Western blot - Anti-IL-33 antibody [EPR20417] (ab207737) at 1/1000 dilution

All lanes:

human IL33 recombinant protein (aa112-270), 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 30 kDa

Observed band size: 18 kDa

false

Exposure time: 2s